{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Johnson ST"],"funding":["the Robert Welch Foundation","NEI NIH HHS","National Institutes of Health","Rusty Kelley Professorship in Medical Science","NIH predoctoral fellowship","NIGMS NIH HHS"],"pagination":["1004-1016"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8370741"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["27(9)"],"pubmed_abstract":["TNRC6 is a scaffolding protein that bridges interactions between small RNAs, argonaute (AGO) protein, and effector proteins to control gene expression. There are three paralogs in mammalian cells, <i>TNRC6A</i>, <i>TNRC6B</i>, and <i>TNRC6C</i> These paralogs have ∼40% amino acid sequence identity and the extent of their unique or redundant functions is unclear. Here, we use knockout cell lines, enhanced crosslinking immunoprecipitation (eCLIP), and high-throughput RNA sequencing (RNA-seq) to explore the roles of TNRC6 paralogs in RNA-mediated control of gene expression. We find that the paralogs are largely functionally redundant and changes in levels of gene expression are well-correlated with those observed in <i>AGO</i> knockout cell lines. Splicing changes observed in <i>AGO</i> knockout cell lines are also observed in <i>TNRC6</i> knockout cells. These data further define the roles of the TNRC6 isoforms as part of the RNA interference (RNAi) machinery."],"journal":["RNA (New York, N.Y.)"],"pubmed_title":["Impact of scaffolding protein TNRC6 paralogs on gene expression and splicing."],"pmcid":["PMC8370741"],"funding_grant_id":["F31 EY030336","GM106151","I-1244","R35 GM118103","5 F31 EY030336-03","R01 GM106151"],"pubmed_authors":["Johnson ST","Liu J","Corey DR","Chu Y"],"additional_accession":[]},"is_claimable":false,"name":"Impact of scaffolding protein TNRC6 paralogs on gene expression and splicing.","description":"TNRC6 is a scaffolding protein that bridges interactions between small RNAs, argonaute (AGO) protein, and effector proteins to control gene expression. There are three paralogs in mammalian cells, <i>TNRC6A</i>, <i>TNRC6B</i>, and <i>TNRC6C</i> These paralogs have ∼40% amino acid sequence identity and the extent of their unique or redundant functions is unclear. Here, we use knockout cell lines, enhanced crosslinking immunoprecipitation (eCLIP), and high-throughput RNA sequencing (RNA-seq) to explore the roles of TNRC6 paralogs in RNA-mediated control of gene expression. We find that the paralogs are largely functionally redundant and changes in levels of gene expression are well-correlated with those observed in <i>AGO</i> knockout cell lines. Splicing changes observed in <i>AGO</i> knockout cell lines are also observed in <i>TNRC6</i> knockout cells. These data further define the roles of the TNRC6 isoforms as part of the RNA interference (RNAi) machinery.","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Sep","modification":"2024-11-05T22:40:19.17Z","creation":"2022-02-11T10:05:39.015Z"},"accession":"S-EPMC8370741","cross_references":{"pubmed":["34108231"],"doi":["10.1261/rna.078709.121"]}}