biostudies-literatureUnknown12(1)Liang CAs a basic structure of most polypyridinal metal complexes, [Ru(bpy)₃]²⁺, has the advantages of simple structure, facile synthesis and high yield, which has great potential for scientific research and application. However, sonodynamic therapy (SDT) performance of [Ru(bpy)₃]²⁺ has not been investigated so far. SDT can overcome the tissue-penetration and phototoxicity problems compared to photodynamic therapy. Here, we report that [Ru(bpy)₃]²⁺ is a highly potent sonosensitizer and sonocatalyst for sonotherapy in vitro and in vivo. [Ru(bpy)₃]²⁺ can produce singlet oxygen (¹O₂) and sono-oxidize endogenous 1,4-dihydronicotinamide adenine dinucleotide (NADH) under ultrasound (US) stimulaNature communications5001https://www.ebi.ac.uk/biostudies/studies/S-EPMC8373944biostudies-literatureA highly potent ruthenium(II)-sonosensitizer and sonocatalyst for in vivo sonotherapy.PMC8373944Liang CHuang CXie JHuang HLuo SZhang QZhang PfalseA highly potent ruthenium(II)-sonosensitizer and sonocatalyst for in vivo sonotherapy.As a basic structure of most polypyridinal metal complexes, [Ru(bpy)₃]²⁺, has the advantages of simple structure, facile synthesis and high yield, which has great potential for scientific research and application. However, sonodynamic therapy (SDT) performance of [Ru(bpy)₃]²⁺ has not been investigated so far. SDT can overcome the tissue-penetration and phototoxicity problems compared to photodynamic therapy. Here, we report that [Ru(bpy)₃]²⁺ is a highly potent sonosensitizer and sonocatalyst for sonotherapy in vitro and in vivo. [Ru(bpy)₃]²⁺ can produce singlet oxygen (¹O₂) and sono-oxidize endogenous 1,4-dihydronicotinamide adenine dinucleotide (NADH) under ultrasound (US) stimula2021-01-01T00:00:00Z2021 Aug2026-05-09T02:26:38.794Z2026-04-08T00:35:25.627ZS-EPMC83739443440815110.1038/s41467-021-25303-1