{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Lebreton S"],"funding":["Associazione Italiana per la Ricerca sul Cancro","Telethon","Agence Nationale de la Recherche","Fondation pour la Recherche Médicale"],"pagination":["e2014709118"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8379914"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["118(33)"],"pubmed_abstract":["Glycosylphosphatidylinositol-anchored proteins (GPI-APs) are lipid-associated luminal secretory cargoes selectively sorted to the apical surface of the epithelia where they reside and play diverse vital functions. Cholesterol-dependent clustering of GPI-APs in the Golgi is the key step driving their apical sorting and their further plasma membrane organization and activity; however, the specific machinery involved in this Golgi event is still poorly understood. In this study, we show that the formation of GPI-AP homoclusters (made of single GPI-AP species) in the Golgi relies directly on the levels of calcium within cisternae. We further demonstrate that the TGN calcium/manganese pump, SPCA1, which regulates the calcium concentration within the Golgi, and Cab45, a calcium-binding luminal G"],"journal":["Proceedings of the National Academy of Sciences of the United States of America"],"pubmed_title":["Calcium levels in the Golgi complex regulate clustering and apical sorting of GPI-APs in polarized epithelial cells."],"pmcid":["PMC8379914"],"funding_grant_id":["IG-14442","GGP15219/B","ANR-16-CE16-0019-01","GGP15219","DEQ20140329557"],"pubmed_authors":["Nitti M","Paladino S","Pinton P","von Blume J","Liu D","Lebreton S","Zurzolo C"],"additional_accession":[]},"is_claimable":false,"name":"Calcium levels in the Golgi complex regulate clustering and apical sorting of GPI-APs in polarized epithelial cells.","description":"Glycosylphosphatidylinositol-anchored proteins (GPI-APs) are lipid-associated luminal secretory cargoes selectively sorted to the apical surface of the epithelia where they reside and play diverse vital functions. Cholesterol-dependent clustering of GPI-APs in the Golgi is the key step driving their apical sorting and their further plasma membrane organization and activity; however, the specific machinery involved in this Golgi event is still poorly understood. In this study, we show that the formation of GPI-AP homoclusters (made of single GPI-AP species) in the Golgi relies directly on the levels of calcium within cisternae. We further demonstrate that the TGN calcium/manganese pump, SPCA1, which regulates the calcium concentration within the Golgi, and Cab45, a calcium-binding luminal G","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Aug","modification":"2025-04-05T14:05:41.812Z","creation":"2025-04-05T14:05:41.812Z"},"accession":"S-EPMC8379914","cross_references":{"pubmed":["34389665"],"doi":["10.1073/pnas.2014709118"]}}