{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Clark SL"],"funding":["NCATS NIH HHS","NIMH NIH HHS","NIAAA NIH HHS","National Institutes of Health"],"pagination":["1524-1532"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8380262"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["60(12)"],"pubmed_abstract":["<h4>Objective</h4>The impact of adolescent cannabis use is a pressing public health question owing to the high rates of use and links to negative outcomes. This study considered the association between problematic adolescent cannabis use and methylation.<h4>Method</h4>Using an enrichment-based sequencing approach, a methylome-wide association study (MWAS) was performed of problematic adolescent cannabis use in 703 adolescent samples from the Great Smoky Mountain Study. Using epigenomic deconvolution, MWASs were performed for the main cell types in blood: granulocytes, T cells, B cells, and monocytes. Enrichment testing was conducted to establish overlap between cannabis-associated methylation differences and variants associated with negative mental health effects of adolescent cannabis use.<h4>Results</h4>Whole-blood analyses identified 45 significant CpGs, and cell type-specific analyses yielded 32 additional CpGs not identified in the whole-blood MWAS. Significant overlap was observed between the B-cell MWAS and genetic studies of education attainment and intelligence. Furthermore, the results from both T cells and monocytes overlapped with findings from an MWAS of psychosis conducted in brain tissue.<h4>Conclusion</h4>In one of the first methylome-wide association studies of adolescent cannabis use, several methylation sites located in genes of importance for potentially relevant brain functions were identified. These findings resulted in several testable hypotheses by which cannabis-associated methylation can impact neurological development and inflammation response as well as potential mechanisms linking cannabis-associated methylation to potential downstream mental health effects."],"journal":["Journal of the American Academy of Child and Adolescent Psychiatry"],"pubmed_title":["Methylomic Investigation of Problematic Adolescent Cannabis Use and Its Negative Mental Health Consequences."],"pmcid":["PMC8380262"],"funding_grant_id":["R01 AA026057","1R01AA026057","1R01MH104576","UL1 TR001863","R01 MH104576"],"pubmed_authors":["Zhao M","Aberg KA","Clark SL","Xie LY","van den Oord EJCG","Chan R","Copeland WE"],"additional_accession":[]},"is_claimable":false,"name":"Methylomic Investigation of Problematic Adolescent Cannabis Use and Its Negative Mental Health Consequences.","description":"<h4>Objective</h4>The impact of adolescent cannabis use is a pressing public health question owing to the high rates of use and links to negative outcomes. This study considered the association between problematic adolescent cannabis use and methylation.<h4>Method</h4>Using an enrichment-based sequencing approach, a methylome-wide association study (MWAS) was performed of problematic adolescent cannabis use in 703 adolescent samples from the Great Smoky Mountain Study. Using epigenomic deconvolution, MWASs were performed for the main cell types in blood: granulocytes, T cells, B cells, and monocytes. Enrichment testing was conducted to establish overlap between cannabis-associated methylation differences and variants associated with negative mental health effects of adolescent cannabis use.<h4>Results</h4>Whole-blood analyses identified 45 significant CpGs, and cell type-specific analyses yielded 32 additional CpGs not identified in the whole-blood MWAS. Significant overlap was observed between the B-cell MWAS and genetic studies of education attainment and intelligence. Furthermore, the results from both T cells and monocytes overlapped with findings from an MWAS of psychosis conducted in brain tissue.<h4>Conclusion</h4>In one of the first methylome-wide association studies of adolescent cannabis use, several methylation sites located in genes of importance for potentially relevant brain functions were identified. These findings resulted in several testable hypotheses by which cannabis-associated methylation can impact neurological development and inflammation response as well as potential mechanisms linking cannabis-associated methylation to potential downstream mental health effects.","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Dec","modification":"2025-04-04T19:38:11.15Z","creation":"2025-04-04T19:38:11.15Z"},"accession":"S-EPMC8380262","cross_references":{"pubmed":["33631312"],"doi":["10.1016/j.jaac.2021.02.008"]}}