biostudies-literatureHe JZhejiang University Student Science and Technology Innovation Activity Plan2708-2709https://www.ebi.ac.uk/biostudies/studies/S-EPMC8382013biostudies-literatureUnknown6(9)In this study, we determined the complete mitochondrial genome of <i>Neolissochilus heterostomus</i>. The genome is 16,585 bp in length, including 2 ribosomal RNA genes, 13 proteins-coding genes, 22 transfer RNA genes, and two non-coding control regions. Sequence analysis showed that the overall base composition of <i>N. heterostomus</i> is T 24.8%, C 27.7%, A 31.7%, and G 15.8%. The sequence is a slight A + T bias of 56.5%, which is similar to other fishes. We describe a phylogenetic analysis of 16 species of Cypriniformes based on the complete mitochondrial genome, and the result showed that <i>N. stracheyi</i> is most closely related to <i>N. heterostomus</i>. This mitogenome sequence data would play an important role in the investigation of phylogenetic relationship of the Cyprinidae.Mitochondrial DNA. Part B, ResourcesCompletely mitochondrial genome of <i>Neolissochilus heterostomus</i>.PMC83820132020R411009Zhao CHe JGuo YZhao BZhang HChu ZfalseCompletely mitochondrial genome of <i>Neolissochilus heterostomus</i>.In this study, we determined the complete mitochondrial genome of <i>Neolissochilus heterostomus</i>. The genome is 16,585 bp in length, including 2 ribosomal RNA genes, 13 proteins-coding genes, 22 transfer RNA genes, and two non-coding control regions. Sequence analysis showed that the overall base composition of <i>N. heterostomus</i> is T 24.8%, C 27.7%, A 31.7%, and G 15.8%. The sequence is a slight A + T bias of 56.5%, which is similar to other fishes. We describe a phylogenetic analysis of 16 species of Cypriniformes based on the complete mitochondrial genome, and the result showed that <i>N. stracheyi</i> is most closely related to <i>N. heterostomus</i>. This mitogenome sequence data would play an important role in the investigation of phylogenetic relationship of the Cyprinidae.2021-01-01T00:00:00Z20212025-05-29T20:28:39.953Z2022-02-11T09:50:29.335ZS-EPMC83820133443512810.1080/23802359.2021.1945966