{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Geng S"],"funding":["NIDCR NIH HHS","National Institute of Dental and Craniofacial Research"],"pagination":["1696-1704"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8386916"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["73(6)"],"pubmed_abstract":["Amelogenin is the most abundant matrix protein guiding hydroxyapatite formation in enamel, the durable bioceramic tissue that covers vertebrate teeth. Here, we sought to refine structure-function for an amelogenin domain based on <i>in vitro</i> data showing a 42 amino acid amelogenin-derived peptide (ADP7) mimicked formation of hydroxyapatite similar to that observed for the full-length mouse 180 amino acid protein. In mice, we used CRISPR-Cas9 to express only ADP7 by the native amelogenin promoter. Analysis revealed ADP7 messenger RNA expression in developing mouse teeth with the formation of a thin layer of enamel. <i>In vivo</i>, ADP7 peptide partially replaced the function of the full-length amelogenin protein and its several protein isoforms. Protein structure-function relationships "],"journal":["JOM (Warrendale, Pa. : 1989)"],"pubmed_title":["Minimal amelogenin domain for enamel formation."],"pmcid":["PMC8386916"],"funding_grant_id":["R37 DE013045","R37 DE13045"],"pubmed_authors":["Geng S","Lei Y","Snead ML"],"additional_accession":[]},"is_claimable":false,"name":"Minimal amelogenin domain for enamel formation.","description":"Amelogenin is the most abundant matrix protein guiding hydroxyapatite formation in enamel, the durable bioceramic tissue that covers vertebrate teeth. Here, we sought to refine structure-function for an amelogenin domain based on <i>in vitro</i> data showing a 42 amino acid amelogenin-derived peptide (ADP7) mimicked formation of hydroxyapatite similar to that observed for the full-length mouse 180 amino acid protein. In mice, we used CRISPR-Cas9 to express only ADP7 by the native amelogenin promoter. Analysis revealed ADP7 messenger RNA expression in developing mouse teeth with the formation of a thin layer of enamel. <i>In vivo</i>, ADP7 peptide partially replaced the function of the full-length amelogenin protein and its several protein isoforms. Protein structure-function relationships ","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Jun","modification":"2025-04-04T20:15:42.639Z","creation":"2025-04-04T20:15:42.639Z"},"accession":"S-EPMC8386916","cross_references":{"pubmed":["34456537"],"doi":["10.1007/s11837-021-04687-x"]}}