biostudies-literatureLi CInterdisciplinary Program of Shanghai Jiao Tong UniversityScience and Technology Commission of Shanghai MunicipalityScience and Technology Commission of Shanghai Municipality (Shanghai Municipal Science and Technology Commission)Nurture projects for basic research of Shanghai Chest Hospital807https://www.ebi.ac.uk/biostudies/studies/S-EPMC8387484biostudies-literatureUnknown12(9)The relationship between circular RNA (circRNA) and cancer stem cells (CSCs) is uncertain. We have investigated the combined influence of CSCs, circRNA (hsa_circ_0003222), and immune checkpoint inhibitors in NSCLC progression and therapy resistance. We constructed lung CSCs (LCSCs; PC9 and A549). The effects of hsa_circ_0003222 in vitro were determined by cell counting, colony and sphere formation, and Transwell assays. A tumor xenograft model of metastasis and orthotopic model were built for in vivo analysis. We found that hsa_circ_0003222 was highly expressed in NSCLC tissues and LCSCs. Higher levels of hsa_circ_0003222 were associated with the stage, metastasis, and survival rate of patients with NSCLC. Reduced levels of hsa_circ_0003222 decreased tumor cell proliferation, migration, inCell death & diseasehsa_circ_0003222 accelerates stemness and progression of non-small cell lung cancer by sponging miR-527.PMC838748418441904700Zheng XLi CZhang JChu TZhang HXu JYang XHu FHu CHan BZhong RPan FLu JZhang XShen YNie Wfalsehsa_circ_0003222 accelerates stemness and progression of non-small cell lung cancer by sponging miR-527.The relationship between circular RNA (circRNA) and cancer stem cells (CSCs) is uncertain. We have investigated the combined influence of CSCs, circRNA (hsa_circ_0003222), and immune checkpoint inhibitors in NSCLC progression and therapy resistance. We constructed lung CSCs (LCSCs; PC9 and A549). The effects of hsa_circ_0003222 in vitro were determined by cell counting, colony and sphere formation, and Transwell assays. A tumor xenograft model of metastasis and orthotopic model were built for in vivo analysis. We found that hsa_circ_0003222 was highly expressed in NSCLC tissues and LCSCs. Higher levels of hsa_circ_0003222 were associated with the stage, metastasis, and survival rate of patients with NSCLC. Reduced levels of hsa_circ_0003222 decreased tumor cell proliferation, migration, in2021-01-01T00:00:00Z2021 Aug2025-05-29T22:30:39.139Z2025-05-29T22:30:39.139ZS-EPMC83874843443381010.1038/s41419-021-04095-8