biostudies-literatureRodrigues-Amorim DFundação para a Ciência e a TecnologiaInstituto de Salud Carlos IIIAxencia Galega de Innovación8499https://www.ebi.ac.uk/biostudies/studies/S-EPMC8395201biostudies-literatureUnknown22(16)The neurobiology of schizophrenia is multifactorial, comprising the dysregulation of several biochemical pathways and molecules. This research proposes a peripheral biomarker for schizophrenia that involves the second extracellular loop of norepinephrine transporter (NEText), the tropomyosin receptor kinase C (TrkC), and the neurotrophin-3 (NT-3) in T cells. The study of NEText, NT-3, and TrkC was performed in T cells and plasma extracted from peripheral blood of 54 patients with schizophrenia and 54 healthy controls. Levels of NT-3, TrkC, and NET were significantly lower in plasma and T cells of patients compared to healthy controls. Co-immunoprecipitation (co-IPs) showed protein interactions with Co-IP NEText-NT-3 and Co-IP NEText-TrkC. Computational modelling of protein-peptide docking International journal of molecular sciencesThe Role of the Second Extracellular Loop of Norepinephrine Transporter, Neurotrophin-3 and Tropomyosin Receptor Kinase C in T Cells: A Peripheral Biomarker in the Etiology of Schizophrenia.PMC8395201P16/00405SFRH/BD/135623/2018IN607B 2018/17PI20/00937Garcia-Caballero ASpuch CLopez-Garcia MRodrigues-Amorim DAlvarez-Ariza MOlivares JMFreiria-Martinez LRodriguez-Jamardo CComis-Tuche Mde Las Heras EVallejo-Curto MDCFernandez-Palleiro PIglesias-Martinez-Almeida MRivera-Baltanas TfalseThe Role of the Second Extracellular Loop of Norepinephrine Transporter, Neurotrophin-3 and Tropomyosin Receptor Kinase C in T Cells: A Peripheral Biomarker in the Etiology of Schizophrenia.The neurobiology of schizophrenia is multifactorial, comprising the dysregulation of several biochemical pathways and molecules. This research proposes a peripheral biomarker for schizophrenia that involves the second extracellular loop of norepinephrine transporter (NEText), the tropomyosin receptor kinase C (TrkC), and the neurotrophin-3 (NT-3) in T cells. The study of NEText, NT-3, and TrkC was performed in T cells and plasma extracted from peripheral blood of 54 patients with schizophrenia and 54 healthy controls. Levels of NT-3, TrkC, and NET were significantly lower in plasma and T cells of patients compared to healthy controls. Co-immunoprecipitation (co-IPs) showed protein interactions with Co-IP NEText-NT-3 and Co-IP NEText-TrkC. Computational modelling of protein-peptide docking 2021-01-01T00:00:00Z2021 Aug2026-04-08T07:46:31.426Z2022-02-11T09:56:57.119ZS-EPMC83952013444520510.3390/ijms22168499