{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Spencer DM"],"funding":["National Institutes of Health","U.S. Department of Veterans Affairs"],"pagination":["8931"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8396244"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["22(16)"],"pubmed_abstract":["DNA is a polymeric macromolecule that can display a variety of backbone conformations. While the classical B-DNA is a right-handed double helix, Z-DNA is a left-handed helix with a zig-zag orientation. The Z conformation depends upon the base sequence, base modification and supercoiling and is considered to be transient. To determine whether the presence of Z-DNA can be detected immunochemically, the binding of monoclonal and polyclonal anti-Z-DNA antibodies to a panel of natural DNA antigens was assessed by an ELISA using brominated poly(dG-dC) as a control for Z-DNA. As these studies showed, among natural DNA tested (Micrococcus luteus, calf thymus, Escherichiacoli, salmon sperm, lambda phage), micrococcal (MC) DNA showed the highest binding with both anti-Z-DNA preparations, and E. coli"],"journal":["International journal of molecular sciences"],"pubmed_title":["The Binding of Monoclonal and Polyclonal Anti-Z-DNA Antibodies to DNA of Various Species Origin."],"pmcid":["PMC8396244"],"funding_grant_id":["R01AR073935","BX003772"],"pubmed_authors":["Pisetsky DS","Reyna AG","Spencer DM"],"additional_accession":[]},"is_claimable":false,"name":"The Binding of Monoclonal and Polyclonal Anti-Z-DNA Antibodies to DNA of Various Species Origin.","description":"DNA is a polymeric macromolecule that can display a variety of backbone conformations. While the classical B-DNA is a right-handed double helix, Z-DNA is a left-handed helix with a zig-zag orientation. The Z conformation depends upon the base sequence, base modification and supercoiling and is considered to be transient. To determine whether the presence of Z-DNA can be detected immunochemically, the binding of monoclonal and polyclonal anti-Z-DNA antibodies to a panel of natural DNA antigens was assessed by an ELISA using brominated poly(dG-dC) as a control for Z-DNA. As these studies showed, among natural DNA tested (Micrococcus luteus, calf thymus, Escherichiacoli, salmon sperm, lambda phage), micrococcal (MC) DNA showed the highest binding with both anti-Z-DNA preparations, and E. coli","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Aug","modification":"2025-06-01T02:17:49.38Z","creation":"2022-02-11T10:03:46.771Z"},"accession":"S-EPMC8396244","cross_references":{"pubmed":["34445637"],"doi":["10.3390/ijms22168931"]}}