biostudies-literatureOzpinar EWNIAID NIH HHSNIEHS NIH HHSNIH HHS1008-1022https://www.ebi.ac.uk/biostudies/studies/S-EPMC8403210biostudies-literatureUnknown27(15-16)Mast cells (MCs) are pro-inflammatory tissue-resident immune cells that play a key role in inflammation. MCs circulate in peripheral blood as progenitors and undergo terminal differentiation in the tissue microenvironment where they can remain for many years. This <i>in situ</i> maturation results in tissue- and species-specific MC phenotypes, culminating in significant variability in response to environmental stimuli. There are many challenges associated with studying mature tissue-derived MCs, particularly in humans. In cases where cultured MCs are able to differentiate in two-dimensional <i>in vitro</i> cultures, there remains an inability for full maturation. Extracellular matrix (ECM) scaffolds provide for a more physiologically relevant environment for cells <i>in vitro</i> and have Tissue engineering. Part ADermal Extracellular Matrix-Derived Hydrogels as an <i>In Vitro</i> Substrate to Study Mast Cell Maturation.PMC8403210R01 AI143985T32 OD011130P30 ES025128Arthur GKFreytes DOMora-Navarro CFrey ALBiehl ASnider DBOzpinar EWCruse GfalseDermal Extracellular Matrix-Derived Hydrogels as an <i>In Vitro</i> Substrate to Study Mast Cell Maturation.Mast cells (MCs) are pro-inflammatory tissue-resident immune cells that play a key role in inflammation. MCs circulate in peripheral blood as progenitors and undergo terminal differentiation in the tissue microenvironment where they can remain for many years. This <i>in situ</i> maturation results in tissue- and species-specific MC phenotypes, culminating in significant variability in response to environmental stimuli. There are many challenges associated with studying mature tissue-derived MCs, particularly in humans. In cases where cultured MCs are able to differentiate in two-dimensional <i>in vitro</i> cultures, there remains an inability for full maturation. Extracellular matrix (ECM) scaffolds provide for a more physiologically relevant environment for cells <i>in vitro</i> and have 2021-01-01T00:00:00Z2021 Aug2025-04-04T13:58:41.684Z2024-11-07T08:11:04.917ZS-EPMC84032103300398210.1089/ten.tea.2020.014210.1089/ten.TEA.2020.0142