biostudies-literatureDing LNatural Science Foundation of Beijing MunicipalityNational Natural Science Foundation of China875-884https://www.ebi.ac.uk/biostudies/studies/S-EPMC8403591biostudies-literatureUnknown10(4)Cervical cancer (CC) is a type of pelvic malignant tumor that severely threatens women's health. Current evidence suggests that <i>IER5</i>, as a potential radiosensitizer, promotes irradiation-induced apoptosis in CC tissues in patients undergoing chemoradiotherapy. <i>IER5</i> has been shown to be involved in the G₂/M-phase transition. In the present study, we used <i>Cdc25B</i> as the breakthrough point to explore the underlying mechanism of <i>IER5</i> in the cell cycle regulation of radiation-damaged HeLa cells. <i>IER5</i> was evidently upregulated after irradiation, but <i>Cdc25B</i> was significantly downregulated. In monoclonal <i>IER5</i>-silenced HeLa cells, irradiation-induced downregulation of <i>Cdc25B</i> was attenuated. The effect of irradiation on <i>Cdc25B</i> Toxicology research<i>Cdc25B</i> is transcriptionally inhibited by <i>IER5</i> through the <i>NF-YB</i> transcription factor in irradiation-treated HeLa cells.PMC840359131640022317709077172146Liu XZhou PJiang XXiong QDing KDing LZhao Xfalse<i>Cdc25B</i> is transcriptionally inhibited by <i>IER5</i> through the <i>NF-YB</i> transcription factor in irradiation-treated HeLa cells.Cervical cancer (CC) is a type of pelvic malignant tumor that severely threatens women's health. Current evidence suggests that <i>IER5</i>, as a potential radiosensitizer, promotes irradiation-induced apoptosis in CC tissues in patients undergoing chemoradiotherapy. <i>IER5</i> has been shown to be involved in the G₂/M-phase transition. In the present study, we used <i>Cdc25B</i> as the breakthrough point to explore the underlying mechanism of <i>IER5</i> in the cell cycle regulation of radiation-damaged HeLa cells. <i>IER5</i> was evidently upregulated after irradiation, but <i>Cdc25B</i> was significantly downregulated. In monoclonal <i>IER5</i>-silenced HeLa cells, irradiation-induced downregulation of <i>Cdc25B</i> was attenuated. The effect of irradiation on <i>Cdc25B</i> 2021-01-01T00:00:00Z2021 Aug2026-04-08T08:43:32.246Z2022-08-04T07:10:47.385ZS-EPMC84035913448467910.1093/toxres/tfab069