{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wise SA"],"funding":["Intramural NIH HHS","National Institutes of Health Office of the Director"],"pagination":["105917"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8403635"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["212"],"pubmed_abstract":["An intralaboratory study assessing assay variability and bias for determination of serum total 25-hydroxyvitamin D [25(OH)D] was conducted by the Vitamin D Standardization Program (VDSP). Thirteen assays for serum total 25(OH)D were evaluated in a single laboratory including 11 unique immunoassays and one liquid chromatography - tandem mass spectrometry (LC-MS/MS) assay. Fifty single-donor serum samples, including eight samples with high concentrations of 25(OH)D<sub>2</sub> (> 30 nmol/L), were assigned target values for 25(OH)D<sub>2</sub> and 25(OH)D<sub>3</sub> using reference measurement procedures (RMP). Using four replicate measurements for each sample, the mean total percent coefficient of variation (%CV) and mean % bias from the target values were determined for each assay using th"],"journal":["The Journal of steroid biochemistry and molecular biology"],"pubmed_title":["Vitamin D Standardization Program (VDSP) intralaboratory study for the assessment of 25-hydroxyvitamin D assay variability and bias."],"pmcid":["PMC8403635"],"funding_grant_id":["Z99 OD999999"],"pubmed_authors":["Camara JE","Hahm G","Sempos CT","Wise SA","Burdette CQ","Cavalier E","Merkel J","Nalin F","Peeters S","Le Goff C","Durazo-Arvizu RA","Lukas P","Kuszak AJ"],"additional_accession":[]},"is_claimable":false,"name":"Vitamin D Standardization Program (VDSP) intralaboratory study for the assessment of 25-hydroxyvitamin D assay variability and bias.","description":"An intralaboratory study assessing assay variability and bias for determination of serum total 25-hydroxyvitamin D [25(OH)D] was conducted by the Vitamin D Standardization Program (VDSP). Thirteen assays for serum total 25(OH)D were evaluated in a single laboratory including 11 unique immunoassays and one liquid chromatography - tandem mass spectrometry (LC-MS/MS) assay. Fifty single-donor serum samples, including eight samples with high concentrations of 25(OH)D<sub>2</sub> (> 30 nmol/L), were assigned target values for 25(OH)D<sub>2</sub> and 25(OH)D<sub>3</sub> using reference measurement procedures (RMP). Using four replicate measurements for each sample, the mean total percent coefficient of variation (%CV) and mean % bias from the target values were determined for each assay using th","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Sep","modification":"2025-04-04T09:37:37.277Z","creation":"2025-04-04T09:37:37.277Z"},"accession":"S-EPMC8403635","cross_references":{"pubmed":["34010687"],"doi":["10.1016/j.jsbmb.2021.105917"]}}