biostudies-literatureUnknown25Hosen MRDeutsche ForschungsgemeinschaftDeutsche Gesellschaft fur Kardiologie - Herz- und Kreislaufforschung eVCorona FoundationLong noncoding RNAs (lncRNAs) have emerged as biomarkers and regulators of cardiovascular disease. However, the expression pattern of circulating extracellular vesicle (EV)-incorporated lncRNAs in patients with coronary artery disease (CAD) is still poorly investigated. A human lncRNA array revealed that certain EV-lncRNAs are significantly dysregulated in CAD patients. Circulating small EVs (sEVs) from patients with (n = 30) or without (n = 30) CAD were used to quantify <i>PUNISHER</i> (also known as <i>AGAP2-</i>antisense RNA 1 [<i>AS1</i>]), <i>GAS5</i>, <i>MALAT1</i>, and <i>H19</i> RNA levels. <i>PUNISHER</i> (p = 0.002) and <i>GAS5</i> (p = 0.02) were significantly increased in patients with CAD, compared to non-CAD patients. Fluorescent labeling and quantitative real-time PCR of sEVMolecular therapy. Nucleic acids388-405https://www.ebi.ac.uk/biostudies/studies/S-EPMC8403722biostudies-literatureCAD increases the long noncoding RNA <i>PUNISHER</i> in small extracellular vesicles and regulates endothelial cell function via vesicular shuttling.PMC8403722Liu YZietzer AGoody PUchida SWerner NHosen MRJansen FNickenig GLi QLatz EMaus KfalseCAD increases the long noncoding RNA <i>PUNISHER</i> in small extracellular vesicles and regulates endothelial cell function via vesicular shuttling.Long noncoding RNAs (lncRNAs) have emerged as biomarkers and regulators of cardiovascular disease. However, the expression pattern of circulating extracellular vesicle (EV)-incorporated lncRNAs in patients with coronary artery disease (CAD) is still poorly investigated. A human lncRNA array revealed that certain EV-lncRNAs are significantly dysregulated in CAD patients. Circulating small EVs (sEVs) from patients with (n = 30) or without (n = 30) CAD were used to quantify <i>PUNISHER</i> (also known as <i>AGAP2-</i>antisense RNA 1 [<i>AS1</i>]), <i>GAS5</i>, <i>MALAT1</i>, and <i>H19</i> RNA levels. <i>PUNISHER</i> (p = 0.002) and <i>GAS5</i> (p = 0.02) were significantly increased in patients with CAD, compared to non-CAD patients. Fluorescent labeling and quantitative real-time PCR of sEV2021-01-01T00:00:00Z2021 Sep2025-04-19T23:58:42.641Z2022-02-11T11:19:15.815ZS-EPMC84037223448486410.1016/j.omtn.2021.05.023