biostudies-literatureLazo EODOE Office of Biological and Environmental ResearchNational Institutes of Health, National Institute of General Medical SciencesNIGMS NIH HHS1649-1661https://www.ebi.ac.uk/biostudies/studies/S-EPMC8415329biostudies-literatureUnknown28(Pt 5)Here we present two robotic sample changers integrated into the experimental stations for the macromolecular crystallography (MX) beamlines AMX and FMX, and the biological small-angle scattering (bioSAXS) beamline LiX. They enable fully automated unattended data collection and remote access to the beamlines. The system designs incorporate high-throughput, versatility, high-capacity, resource sharing and robustness. All systems are centered around a six-axis industrial robotic arm coupled with a force torque sensor and in-house end effectors (grippers). They have the same software architecture and the facility standard EPICS-based BEAST alarm system. The MX system is compatible with SPINE bases and Unipucks. It comprises a liquid nitrogen dewar holding 384 samples (24 Unipucks) and a stay-cJournal of synchrotron radiationRobotic sample changers for macromolecular X-ray crystallography and biological small-angle X-ray scattering at the National Synchrotron Light Source II.PMC8415329KP1607011P30 GM133893McSweeney SYang LSkinner JJakoncic JBernstein HJLazo EOGuichard NAntonelli SShea-McCarthy GFuchs MRAishima JMyers SSchneider DSweet RBhogadi DQian KfalseRobotic sample changers for macromolecular X-ray crystallography and biological small-angle X-ray scattering at the National Synchrotron Light Source II.Here we present two robotic sample changers integrated into the experimental stations for the macromolecular crystallography (MX) beamlines AMX and FMX, and the biological small-angle scattering (bioSAXS) beamline LiX. They enable fully automated unattended data collection and remote access to the beamlines. The system designs incorporate high-throughput, versatility, high-capacity, resource sharing and robustness. All systems are centered around a six-axis industrial robotic arm coupled with a force torque sensor and in-house end effectors (grippers). They have the same software architecture and the facility standard EPICS-based BEAST alarm system. The MX system is compatible with SPINE bases and Unipucks. It comprises a liquid nitrogen dewar holding 384 samples (24 Unipucks) and a stay-c2021-01-01T00:00:00Z2021 Sep2026-06-02T21:53:34.128Z2025-04-04T09:37:34.853ZS-EPMC84153293447531210.1107/S160057752100757810.1107/s1600577521007578