biostudies-literatureJain RNIBIB NIH HHSU.S. Department of Energy, Office of ScienceAir Force Research LaboratoryCase Western Reserve UniversityNational Institutes of Health, National Institute of Biomedical Imaging and BioengineeringBrookhaven National LaboratoryNational Science Foundation1321-1332https://www.ebi.ac.uk/biostudies/studies/S-EPMC8415340biostudies-literatureUnknown28(Pt 5)Synchrotron X-ray footprinting (XF) is a growing structural biology technique that leverages radiation-induced chemical modifications via X-ray radiolysis of water to produce hydroxyl radicals that probe changes in macromolecular structure and dynamics in solution states of interest. The X-ray Footprinting of Biological Materials (XFP) beamline at the National Synchrotron Light Source II provides the structural biology community with access to instrumentation and expert support in the XF method, and is also a platform for development of new technological capabilities in this field. The design and implementation of a new high-throughput endstation device based around use of a 96-well PCR plate form factor and supporting diagnostic instrumentation for synchrotron XF is described. This develoJournal of synchrotron radiationNew high-throughput endstation to accelerate the experimental optimization pipeline for synchrotron X-ray footprinting.PMC8415340FA8650-18-2-5402DBI-1228549P30 EB009998DE-SC0012704P30-EB-009998Jain RRakitin MAbel DSullivan MChance MRFarquhar ERLodowski DTfalseNew high-throughput endstation to accelerate the experimental optimization pipeline for synchrotron X-ray footprinting.Synchrotron X-ray footprinting (XF) is a growing structural biology technique that leverages radiation-induced chemical modifications via X-ray radiolysis of water to produce hydroxyl radicals that probe changes in macromolecular structure and dynamics in solution states of interest. The X-ray Footprinting of Biological Materials (XFP) beamline at the National Synchrotron Light Source II provides the structural biology community with access to instrumentation and expert support in the XF method, and is also a platform for development of new technological capabilities in this field. The design and implementation of a new high-throughput endstation device based around use of a 96-well PCR plate form factor and supporting diagnostic instrumentation for synchrotron XF is described. This develo2021-01-01T00:00:00Z2021 Sep2026-06-02T21:53:32.882Z2025-04-04T09:37:21.749ZS-EPMC84153403447528110.1107/S160057752100502610.1107/s1600577521005026