{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["11"],"submitter":["Ramirez-Granillo A"],"pubmed_abstract":["<h4>Background</h4>Coinfections with fungi and bacteria in ocular pathologies are increasing at an alarming rate. Two of the main etiologic agents of infections on the corneal surface, such as <i>Aspergillus fumigatus</i> and <i>Staphylococcus aureus</i>, can form a biofilm. However, mixed fungal-bacterial biofilms are rarely reported in ocular infections. The implementation of cell cultures as a study model related to biofilm microbial keratitis will allow understanding the pathogenesis in the cornea. The cornea maintains a pathogen-free ocular surface in which human limbo-corneal fibroblast cells are part of its cell regeneration process. There are no reports of biofilm formation assays on limbo-corneal fibroblasts, as well as their behavior with a polymicrobial infection.<h4>Objective</"],"journal":["Frontiers in cellular and infection microbiology"],"pagination":["646054"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8415486"],"repository":["biostudies-literature"],"pubmed_title":["Microbial Warfare on Three Fronts: Mixed Biofilm of <i>Aspergillus fumigatus</i> and <i>Staphylococcus aureus</i> on Primary Cultures of Human Limbo-Corneal Fibroblasts."],"pmcid":["PMC8415486"],"pubmed_authors":["Rodriguez-Tovar AV","Dominguez-Lopez A","Martinez-Rivera MLA","Bautista-Hernandez LA","Bautista-De Lucio VM","Ramirez-Granillo A","Perez NO","Magana-Guerrero FS","Cordova-Alcantara IM"],"additional_accession":[]},"is_claimable":false,"name":"Microbial Warfare on Three Fronts: Mixed Biofilm of <i>Aspergillus fumigatus</i> and <i>Staphylococcus aureus</i> on Primary Cultures of Human Limbo-Corneal Fibroblasts.","description":"<h4>Background</h4>Coinfections with fungi and bacteria in ocular pathologies are increasing at an alarming rate. Two of the main etiologic agents of infections on the corneal surface, such as <i>Aspergillus fumigatus</i> and <i>Staphylococcus aureus</i>, can form a biofilm. However, mixed fungal-bacterial biofilms are rarely reported in ocular infections. The implementation of cell cultures as a study model related to biofilm microbial keratitis will allow understanding the pathogenesis in the cornea. The cornea maintains a pathogen-free ocular surface in which human limbo-corneal fibroblast cells are part of its cell regeneration process. There are no reports of biofilm formation assays on limbo-corneal fibroblasts, as well as their behavior with a polymicrobial infection.<h4>Objective</","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021","modification":"2025-08-12T03:04:50.372Z","creation":"2022-02-11T10:14:04.163Z"},"accession":"S-EPMC8415486","cross_references":{"pubmed":["34485167"],"doi":["10.3389/fcimb.2021.646054"]}}