biostudies-literatureUnknown11Ramirez-Granillo A<h4>Background</h4>Coinfections with fungi and bacteria in ocular pathologies are increasing at an alarming rate. Two of the main etiologic agents of infections on the corneal surface, such as <i>Aspergillus fumigatus</i> and <i>Staphylococcus aureus</i>, can form a biofilm. However, mixed fungal-bacterial biofilms are rarely reported in ocular infections. The implementation of cell cultures as a study model related to biofilm microbial keratitis will allow understanding the pathogenesis in the cornea. The cornea maintains a pathogen-free ocular surface in which human limbo-corneal fibroblast cells are part of its cell regeneration process. There are no reports of biofilm formation assays on limbo-corneal fibroblasts, as well as their behavior with a polymicrobial infection.<h4>Objective</Frontiers in cellular and infection microbiology646054https://www.ebi.ac.uk/biostudies/studies/S-EPMC8415486biostudies-literatureMicrobial Warfare on Three Fronts: Mixed Biofilm of <i>Aspergillus fumigatus</i> and <i>Staphylococcus aureus</i> on Primary Cultures of Human Limbo-Corneal Fibroblasts.PMC8415486Rodriguez-Tovar AVDominguez-Lopez AMartinez-Rivera MLABautista-Hernandez LABautista-De Lucio VMRamirez-Granillo APerez NOMagana-Guerrero FSCordova-Alcantara IMfalseMicrobial Warfare on Three Fronts: Mixed Biofilm of <i>Aspergillus fumigatus</i> and <i>Staphylococcus aureus</i> on Primary Cultures of Human Limbo-Corneal Fibroblasts.<h4>Background</h4>Coinfections with fungi and bacteria in ocular pathologies are increasing at an alarming rate. Two of the main etiologic agents of infections on the corneal surface, such as <i>Aspergillus fumigatus</i> and <i>Staphylococcus aureus</i>, can form a biofilm. However, mixed fungal-bacterial biofilms are rarely reported in ocular infections. The implementation of cell cultures as a study model related to biofilm microbial keratitis will allow understanding the pathogenesis in the cornea. The cornea maintains a pathogen-free ocular surface in which human limbo-corneal fibroblast cells are part of its cell regeneration process. There are no reports of biofilm formation assays on limbo-corneal fibroblasts, as well as their behavior with a polymicrobial infection.<h4>Objective</2021-01-01T00:00:00Z20212025-08-12T03:04:50.372Z2022-02-11T10:14:04.163ZS-EPMC84154863448516710.3389/fcimb.2021.646054