{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Peterson LS"],"funding":["Doris Duke Charitable Foundation","NIGMS NIH HHS"],"pagination":["714090"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8420969"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12"],"pubmed_abstract":["Although most causes of death and morbidity in premature infants are related to immune maladaptation, the premature immune system remains poorly understood. We provide a comprehensive single-cell depiction of the neonatal immune system at birth across the spectrum of viable gestational age (GA), ranging from 25 weeks to term. A mass cytometry immunoassay interrogated all major immune cell subsets, including signaling activity and responsiveness to stimulation. An elastic net model described the relationship between GA and immunome (R=0.85, p=8.75e-14), and unsupervised clustering highlighted previously unrecognized GA-dependent immune dynamics, including decreasing basal MAP-kinase/NFκB signaling in antigen presenting cells; increasing responsiveness of cytotoxic lymphocytes to interferon-α; and decreasing frequency of regulatory and invariant T cells, including NKT-like cells and CD8<sup>+</sup>CD161<sup>+</sup> T cells. Knowledge gained from the analysis of the neonatal immune landscape across GA provides a mechanistic framework to understand the unique susceptibility of preterm infants to both hyper-inflammatory diseases and infections."],"journal":["Frontiers in immunology"],"pubmed_title":["Single-Cell Analysis of the Neonatal Immune System Across the Gestational Age Continuum."],"pmcid":["PMC8420969"],"funding_grant_id":["2018100","R35 GM137936","R35 GM138353"],"pubmed_authors":["Feyaerts D","Houghteling P","Tsai AS","Stelzer IA","Tsai ES","Han X","Aghaeepour N","Ando K","Lewis DB","Angst MS","Gaudilliere B","Reiss JD","Harbert E","Ringle M","Winn VD","Hedou J","Adusumelli Y","Peterson LS","Ganio EA","Stevenson DK"],"additional_accession":[]},"is_claimable":false,"name":"Single-Cell Analysis of the Neonatal Immune System Across the Gestational Age Continuum.","description":"Although most causes of death and morbidity in premature infants are related to immune maladaptation, the premature immune system remains poorly understood. We provide a comprehensive single-cell depiction of the neonatal immune system at birth across the spectrum of viable gestational age (GA), ranging from 25 weeks to term. A mass cytometry immunoassay interrogated all major immune cell subsets, including signaling activity and responsiveness to stimulation. An elastic net model described the relationship between GA and immunome (R=0.85, p=8.75e-14), and unsupervised clustering highlighted previously unrecognized GA-dependent immune dynamics, including decreasing basal MAP-kinase/NFκB signaling in antigen presenting cells; increasing responsiveness of cytotoxic lymphocytes to interferon-α; and decreasing frequency of regulatory and invariant T cells, including NKT-like cells and CD8<sup>+</sup>CD161<sup>+</sup> T cells. Knowledge gained from the analysis of the neonatal immune landscape across GA provides a mechanistic framework to understand the unique susceptibility of preterm infants to both hyper-inflammatory diseases and infections.","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021","modification":"2026-03-12T23:30:04.178Z","creation":"2025-08-12T03:04:48.97Z"},"accession":"S-EPMC8420969","cross_references":{"pubmed":["34497610"],"doi":["10.3389/fimmu.2021.714090"]}}