{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zhou C"],"funding":["National Institute of Environmental Health Sciences","Shenzhen Engineering Laboratory of Single-molecule Detection and Instrument Development","Guangdong Program","NIEHS NIH HHS","Shenzhen Fundamental Research Program","National Institute of Food and Agriculture"],"pagination":["11424-11432"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8422985"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["93(33)"],"pubmed_abstract":["Enzyme-linked immunosorbent assays (ELISA), as one of the most used immunoassays, have been conducted ubiquitously in hospitals, research laboratories, etc. However, the conventional ELISA procedure is usually laborious, occupies bulky instruments, consumes lengthy operation time, and relies considerably on the skills of technicians, and such limitations call for innovations to develop a fully automated ELISA platform. In this paper, we have presented a system incorporating a robotic-microfluidic interface (RoMI) and a modular hybrid microfluidic chip that embeds a highly sensitive nanofibrous membrane, referred to as the Robotic ELISA, to achieve human-free sample-to-answer ELISA tests in a fully programmable and automated manner. It carries out multiple bioanalytical procedures to replac"],"journal":["Analytical chemistry"],"pubmed_title":["Sample-to-Answer Robotic ELISA."],"pmcid":["PMC8422985"],"funding_grant_id":["5P42ES004699-30","JCYJ 20170413164102261","XMHT20190204002","P42 ES004699","2016ZT06D631","2018-67017-28116/1015597"],"pubmed_authors":["Emami S","Fang Z","Zhao C","Pan T","Taha AY","Zhou C","Mai X","Sun G"],"additional_accession":[]},"is_claimable":false,"name":"Sample-to-Answer Robotic ELISA.","description":"Enzyme-linked immunosorbent assays (ELISA), as one of the most used immunoassays, have been conducted ubiquitously in hospitals, research laboratories, etc. However, the conventional ELISA procedure is usually laborious, occupies bulky instruments, consumes lengthy operation time, and relies considerably on the skills of technicians, and such limitations call for innovations to develop a fully automated ELISA platform. In this paper, we have presented a system incorporating a robotic-microfluidic interface (RoMI) and a modular hybrid microfluidic chip that embeds a highly sensitive nanofibrous membrane, referred to as the Robotic ELISA, to achieve human-free sample-to-answer ELISA tests in a fully programmable and automated manner. It carries out multiple bioanalytical procedures to replac","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Aug","modification":"2025-04-22T09:59:41.018Z","creation":"2025-04-05T23:24:06.014Z"},"accession":"S-EPMC8422985","cross_references":{"pubmed":["34378906"],"doi":["10.1021/acs.analchem.1c01231"]}}