{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Schroeder S"],"funding":["Bundesministerium für Bildung und Forschung (Federal Ministry of Education and Research)","Bundesministerium für Bildung und Forschung"],"pagination":["5324"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8423819"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12(1)"],"pubmed_abstract":["Middle East respiratory syndrome coronavirus (MERS-CoV) is enzootic in dromedary camels across the Middle East and Africa. Virus-induced pneumonia in humans results from animal contact, with a potential for limited onward transmission. Phenotypic changes have been suspected after a novel recombinant clade (lineage 5) caused large nosocomial outbreaks in Saudi Arabia and South Korea in 2016. However, there has been no functional assessment. Here we perform a comprehensive in vitro and ex vivo comparison of viruses from parental and recombinant virus lineages (lineage 3, n = 7; lineage 4, n = 8; lineage 5, n = 9 viruses) from Saudi Arabia, isolated immediately before and after the shift toward lineage 5. Replication of lineage 5 viruses is significantly increased. Transcriptional profiling f"],"journal":["Nature communications"],"pubmed_title":["Functional comparison of MERS-coronavirus lineages reveals increased replicative fitness of the recombinant lineage 5."],"pmcid":["PMC8423819"],"funding_grant_id":["01KI1723A"],"pubmed_authors":["Boulant S","Eggeling S","Wolff T","Hippenstiel S","Mache C","Dijkman R","Thiel V","Schroeder S","Kleine-Weber H","Muth D","Gultom M","Pohlmann S","Muller MA","Fatykhova D","Corman VM","Hocke A","Drosten C","Memish ZA","Stanifer ML","Richter A"],"additional_accession":[]},"is_claimable":false,"name":"Functional comparison of MERS-coronavirus lineages reveals increased replicative fitness of the recombinant lineage 5.","description":"Middle East respiratory syndrome coronavirus (MERS-CoV) is enzootic in dromedary camels across the Middle East and Africa. Virus-induced pneumonia in humans results from animal contact, with a potential for limited onward transmission. Phenotypic changes have been suspected after a novel recombinant clade (lineage 5) caused large nosocomial outbreaks in Saudi Arabia and South Korea in 2016. However, there has been no functional assessment. Here we perform a comprehensive in vitro and ex vivo comparison of viruses from parental and recombinant virus lineages (lineage 3, n = 7; lineage 4, n = 8; lineage 5, n = 9 viruses) from Saudi Arabia, isolated immediately before and after the shift toward lineage 5. Replication of lineage 5 viruses is significantly increased. Transcriptional profiling f","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Sep","modification":"2026-05-09T05:46:36.906Z","creation":"2022-02-11T15:00:09.679Z"},"accession":"S-EPMC8423819","cross_references":{"pubmed":["34493730"],"doi":["10.1038/s41467-021-25519-1"]}}