{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["VanRyzin JW"],"funding":["University of Maryland School of Medicine Dean’s Office","HHS | NIH | National Institute of Mental Health","NICHD NIH HHS","NIDA NIH HHS","NIMH NIH HHS","HHS | NIH | National Institute on Drug Abuse","HHS | NIH | Eunice Kennedy Shriver National Institute of Child Health and Human Development"],"pagination":["ENEURO.0026-21.2021"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8425967"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["8(5)"],"pubmed_abstract":["Neuroscience has been transformed by the ability to genetically modify inbred mice, including the ability to express fluorescent markers specific to cell types or activation states. This approach has been put to particularly good effect in the study of the innate immune cells of the brain, microglia. These specialized macrophages are exceedingly small and complex, but also highly motile and mobile. To date, there have been no tools similar to those in mice available for studying these fundamental cells in the rat brain, and we seek to fill that gap with the generation of the genetically modified Sprague Dawley rat line: <i>SD-Tg(Iba1-EGFP)Mmmc</i> Using CRISPR-Cas/9 technology, we knocked in <i>EGFP</i> to the promoter of the gene <i>Iba1</i> With four male and three female founders confir"],"journal":["eNeuro"],"pubmed_title":["Generation of an Iba1-EGFP Transgenic Rat for the Study of Microglia in an Outbred Rodent Strain."],"pmcid":["PMC8425967"],"funding_grant_id":["R01 MH091424","P01 HD085928","R01 DA039062","F32 HD097816","R01 MH052716"],"pubmed_authors":["Davis KT","Edwards S","Ashton SE","Marquardt AE","Holley A","McCarthy MM","Arambula SE","Blanchard AC","Reinl EL","VanRyzin JW","Pickett LA","Graham EL","Perez-Pouchoulen M","Burzinski MD","Kight KE"],"additional_accession":[]},"is_claimable":false,"name":"Generation of an Iba1-EGFP Transgenic Rat for the Study of Microglia in an Outbred Rodent Strain.","description":"Neuroscience has been transformed by the ability to genetically modify inbred mice, including the ability to express fluorescent markers specific to cell types or activation states. This approach has been put to particularly good effect in the study of the innate immune cells of the brain, microglia. These specialized macrophages are exceedingly small and complex, but also highly motile and mobile. To date, there have been no tools similar to those in mice available for studying these fundamental cells in the rat brain, and we seek to fill that gap with the generation of the genetically modified Sprague Dawley rat line: <i>SD-Tg(Iba1-EGFP)Mmmc</i> Using CRISPR-Cas/9 technology, we knocked in <i>EGFP</i> to the promoter of the gene <i>Iba1</i> With four male and three female founders confir","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Sep-Oct","modification":"2026-05-01T03:08:00.003Z","creation":"2022-02-11T12:43:12.06Z"},"accession":"S-EPMC8425967","cross_references":{"pubmed":["34417284"],"doi":["10.1523/ENEURO.0026-21.2021"]}}