biostudies-literatureHelou LLigue Contre le CancerConseil Régional du Centre-Val de LoireDivision of Cancer Prevention, National Cancer InstituteNCI NIH HHSSociété Nationale Française de Gastro-Entérologie166805https://www.ebi.ac.uk/biostudies/studies/S-EPMC8426422biostudies-literatureUnknown433(7)PiggyBac(PB)-like elements (pble) are members of a eukaryotic DNA transposon family. This family is of interest to evolutionary genomics because pble transposases have been domesticated at least 9 times in vertebrates. The amino acid sequence of pble transposases can be split into three regions: an acidic N-terminal domain (~100 aa), a central domain (~400 aa) containing a DD[D/E] catalytic triad, and a cysteine-rich domain (CRD; ~90 aa). Two recent reports suggested that a functional CRD is required for pble transposase activity. Here we found that two CRD-deficient pble transposases, a PB variant and an isoform encoded by the domesticated PB-derived vertebrate transposase gene 5 (pgbd5) trigger transposition of the Ifp2 pble. When overexpressed in HeLa cells, these CRD-deficient transposJournal of molecular biologyThe C-terminal Domain of piggyBac Transposase Is Not Required for DNA Transposition.PMC8426422P30 CA008748R01 CA214812Helou LDardente HGuillou FBeauclair LBuisine NKentsis AJaszczyszyn YArensburger PBigot YLecomte TfalseThe C-terminal Domain of piggyBac Transposase Is Not Required for DNA Transposition.PiggyBac(PB)-like elements (pble) are members of a eukaryotic DNA transposon family. This family is of interest to evolutionary genomics because pble transposases have been domesticated at least 9 times in vertebrates. The amino acid sequence of pble transposases can be split into three regions: an acidic N-terminal domain (~100 aa), a central domain (~400 aa) containing a DD[D/E] catalytic triad, and a cysteine-rich domain (CRD; ~90 aa). Two recent reports suggested that a functional CRD is required for pble transposase activity. Here we found that two CRD-deficient pble transposases, a PB variant and an isoform encoded by the domesticated PB-derived vertebrate transposase gene 5 (pgbd5) trigger transposition of the Ifp2 pble. When overexpressed in HeLa cells, these CRD-deficient transpos2021-01-01T00:00:00Z2021 Apr2026-05-09T03:10:58.138Z2025-02-18T23:36:40.797ZS-EPMC84264223345025310.1016/j.jmb.2020.166805