biostudies-literatureAlfino LNNCATS NIH HHSNIH Office of the DirectorNIH HHS981-993https://www.ebi.ac.uk/biostudies/studies/S-EPMC8426451biostudies-literatureUnknown58(5)Periostin is a matricellular protein important in regulating bone, tooth, and cardiac development. In pathologic conditions, periostin drives allergic and fibrotic inflammatory diseases and is also overexpressed in certain cancers. Periostin signaling in tumors has been shown to promote angiogenesis, metastasis, and cancer stem cell survival in rodent models, and its overexpression is associated with poor prognosis in human glioblastoma. However, the role of periostin in regulating tumorigenesis of canine cancers has not been evaluated. Given its role in bone development, we sought to evaluate mRNA and protein expression of periostin in canine osteosarcoma (OS) and assess its association with patient outcome. We validated an anti-human periostin antibody cross-reactive to canine periostin Veterinary pathologyRole of Periostin Expression in Canine Osteosarcoma Biology and Clinical Outcome.PMC8426451K01 OD022982R03 OD0282651R03OD028265L30 TR002126T35 OD015130Coy JEhrhart EJDuval DLWilczewski-Shirai KCGlapa KCharles JBRegan DPAlfino LNCronise KEfalseRole of Periostin Expression in Canine Osteosarcoma Biology and Clinical Outcome.Periostin is a matricellular protein important in regulating bone, tooth, and cardiac development. In pathologic conditions, periostin drives allergic and fibrotic inflammatory diseases and is also overexpressed in certain cancers. Periostin signaling in tumors has been shown to promote angiogenesis, metastasis, and cancer stem cell survival in rodent models, and its overexpression is associated with poor prognosis in human glioblastoma. However, the role of periostin in regulating tumorigenesis of canine cancers has not been evaluated. Given its role in bone development, we sought to evaluate mRNA and protein expression of periostin in canine osteosarcoma (OS) and assess its association with patient outcome. We validated an anti-human periostin antibody cross-reactive to canine periostin 2021-01-01T00:00:00Z2021 Sep2025-04-04T09:37:36.256Z2025-04-04T09:37:36.256ZS-EPMC84264513368529610.1177/0300985821996671