biostudies-literatureWisnewski AVNCATS NIH HHSNational Institute for Occupational Safety and HealthNIOSH CDC HHSe0252849https://www.ebi.ac.uk/biostudies/studies/S-EPMC8428655biostudies-literatureUnknown16(9)Reverse vaccinology is an evolving approach for improving vaccine effectiveness and minimizing adverse responses by limiting immunizations to critical epitopes. Towards this goal, we sought to identify immunogenic amino acid motifs and linear epitopes of the SARS-CoV-2 spike protein that elicit IgG in COVID-19 mRNA vaccine recipients. Paired pre/post vaccination samples from N = 20 healthy adults, and post-vaccine samples from an additional N = 13 individuals were used to immunoprecipitate IgG targets expressed by a bacterial display random peptide library, and preferentially recognized peptides were mapped to the spike primary sequence. The data identify several distinct amino acid motifs recognized by vaccine-induced IgG, a subset of those targeted by IgG from natural infection, which maPloS oneImmunogenic amino acid motifs and linear epitopes of COVID-19 mRNA vaccines.PMC8428655T03 OH008607OH008607-15UL1 TR001863Santiago RCampillo Luna JLiu JRedlich CAKamath KShon JCHaynes WAFazen LWaitz RBaum-Jones EAbad QASmith RFMartinez BWisnewski AVfalseImmunogenic amino acid motifs and linear epitopes of COVID-19 mRNA vaccines.Reverse vaccinology is an evolving approach for improving vaccine effectiveness and minimizing adverse responses by limiting immunizations to critical epitopes. Towards this goal, we sought to identify immunogenic amino acid motifs and linear epitopes of the SARS-CoV-2 spike protein that elicit IgG in COVID-19 mRNA vaccine recipients. Paired pre/post vaccination samples from N = 20 healthy adults, and post-vaccine samples from an additional N = 13 individuals were used to immunoprecipitate IgG targets expressed by a bacterial display random peptide library, and preferentially recognized peptides were mapped to the spike primary sequence. The data identify several distinct amino acid motifs recognized by vaccine-induced IgG, a subset of those targeted by IgG from natural infection, which ma2021-01-01T00:00:00Z20212025-04-26T10:36:57.678Z2022-02-11T10:36:59.377ZS-EPMC84286553449965210.1371/journal.pone.0252849