{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Swaminathan G"],"funding":["NIDDK NIH HHS","NIAID NIH HHS"],"pagination":["245-259"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8434991"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["132"],"pubmed_abstract":["Human intestinal enteroids (HIE) models have contributed significantly to our understanding of diarrheal diseases and other intestinal infections, but their routine culture conditions fail to mimic the mechanical environment of the native intestinal wall. Because the mechanical characteristics of the intestine significantly alter how pathogens interact with the intestinal epithelium, we used different concentrations of polyethylene glycol (PEG) to generate soft (~2 kPa), medium (~10 kPa), and stiff (~100 kPa) hydrogel biomaterial scaffolds. The height of HIEs cultured in monolayers atop these hydrogels was 18 µm whereas HIEs grown on rigid tissue culture surfaces (with stiffness in the GPa range) were 10 µm. Substrate stiffness also influenced the amount of enteroaggregative E. coli (EAEC "],"journal":["Acta biomaterialia"],"pubmed_title":["Effect of substrate stiffness on human intestinal enteroids' infectivity by enteroaggregative Escherichia coli."],"pmcid":["PMC8434991"],"funding_grant_id":["U19 AI116497","P30 DK056338"],"pubmed_authors":["Rajan A","Huang C","Grande-Allen KJ","Coarfa C","Shannon TE","Carter HE","Karandikar U","Maresso AW","Swaminathan G","Shroyer NF","Kamyabi N","Tadros M","Estes MK","Criss ZK","Robertson MJ"],"additional_accession":[]},"is_claimable":false,"name":"Effect of substrate stiffness on human intestinal enteroids' infectivity by enteroaggregative Escherichia coli.","description":"Human intestinal enteroids (HIE) models have contributed significantly to our understanding of diarrheal diseases and other intestinal infections, but their routine culture conditions fail to mimic the mechanical environment of the native intestinal wall. Because the mechanical characteristics of the intestine significantly alter how pathogens interact with the intestinal epithelium, we used different concentrations of polyethylene glycol (PEG) to generate soft (~2 kPa), medium (~10 kPa), and stiff (~100 kPa) hydrogel biomaterial scaffolds. The height of HIEs cultured in monolayers atop these hydrogels was 18 µm whereas HIEs grown on rigid tissue culture surfaces (with stiffness in the GPa range) were 10 µm. Substrate stiffness also influenced the amount of enteroaggregative E. coli (EAEC ","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Sep","modification":"2025-04-22T21:12:35.287Z","creation":"2025-04-06T03:28:00.068Z"},"accession":"S-EPMC8434991","cross_references":{"pubmed":["34280559"],"doi":["10.1016/j.actbio.2021.07.024"]}}