biostudies-literatureGrant JHansjörg Wyss Institute for Biologically Inspired EngineeringNational Center for Advancing Translational SciencesDefense Advanced Research Projects AgencyNHLBI NIH HHS3509-3519https://www.ebi.ac.uk/biostudies/studies/S-EPMC8440455biostudies-literatureUnknown21(18)Microfluidic organ-on-a-chip (Organ Chip) cell culture devices are often fabricated using polydimethylsiloxane (PDMS) because it is biocompatible, transparent, elastomeric, and oxygen permeable; however, hydrophobic small molecules can absorb to PDMS, which makes it challenging to predict drug responses. Here, we describe a combined simulation and experimental approach to predict the spatial and temporal concentration profile of a drug under continuous dosing in a PDMS Organ Chip containing two parallel channels separated by a porous membrane that is lined with cultured cells, without prior knowledge of its log <i>P</i> value. First, a three-dimensional finite element model of drug loss into the chip was developed that incorporates absorption, adsorption, convection, and diffusion, which sLab on a chipSimulating drug concentrations in PDMS microfluidic organ chips.PMC84404551-UH3-HL-141797-01UH3 HL141797W911NF-16-C-0050HR0011-19-2-0008/AWD-102840-612Ingber DEOzkan APrantil-Baun ROh CMahajan GGrant JfalseSimulating drug concentrations in PDMS microfluidic organ chips.Microfluidic organ-on-a-chip (Organ Chip) cell culture devices are often fabricated using polydimethylsiloxane (PDMS) because it is biocompatible, transparent, elastomeric, and oxygen permeable; however, hydrophobic small molecules can absorb to PDMS, which makes it challenging to predict drug responses. Here, we describe a combined simulation and experimental approach to predict the spatial and temporal concentration profile of a drug under continuous dosing in a PDMS Organ Chip containing two parallel channels separated by a porous membrane that is lined with cultured cells, without prior knowledge of its log <i>P</i> value. First, a three-dimensional finite element model of drug loss into the chip was developed that incorporates absorption, adsorption, convection, and diffusion, which s2021-01-01T00:00:00Z2021 Sep2025-04-04T09:37:18.637Z2025-04-04T09:37:18.637ZS-EPMC84404553434647110.1039/d1lc00348h