{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Sanchez-Cardenas C"],"funding":["Eunice Kennedy Shriver National Institute of Child Health and Human Development","Consejo Nacional de Ciencia y Tecnología","NICHD NIH HHS"],"pagination":["e21528"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8441833"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["35(4)"],"pubmed_abstract":["We have recently reported two different methodologies that improve sperm functionality. The first method involved transient exposure to the Ca<sup>2+</sup> ionophore A<sub>23187</sub> , and the second required sperm incubation in the absence of energy nutrients (starvation). Both methods were associated with an initial loss of motility followed by a rescue step involving ionophore removal or addition of energy metabolites, respectively. In this work, we show that starvation is accompanied by an increase in intracellular Ca<sup>2+</sup> ([Ca<sup>2+</sup> ]<sub>i</sub> ). Additionally, the starved cells acquire a significantly enhanced capacity to undergo a progesterone-induced acrosome reaction. Electrophysiological measurements show that CatSper channel remains active in starvation conditi"],"journal":["FASEB journal : official publication of the Federation of American Societies for Experimental Biology"],"pubmed_title":["Starvation induces an increase in intracellular calcium and potentiates the progesterone-induced mouse sperm acrosome reaction."],"pmcid":["PMC8441833"],"funding_grant_id":["R01 HD088571","HD‐038082, HD‐088571","314839 and Fronteras 71","R01 HD038082"],"pubmed_authors":["Darszon A","Sanchez-Cardenas C","De la Vega-Beltran JL","Hernandez-Cruz A","Martin-Hidalgo D","Orta G","Romarowski A","Visconti PE"],"additional_accession":[]},"is_claimable":false,"name":"Starvation induces an increase in intracellular calcium and potentiates the progesterone-induced mouse sperm acrosome reaction.","description":"We have recently reported two different methodologies that improve sperm functionality. The first method involved transient exposure to the Ca<sup>2+</sup> ionophore A<sub>23187</sub> , and the second required sperm incubation in the absence of energy nutrients (starvation). Both methods were associated with an initial loss of motility followed by a rescue step involving ionophore removal or addition of energy metabolites, respectively. In this work, we show that starvation is accompanied by an increase in intracellular Ca<sup>2+</sup> ([Ca<sup>2+</sup> ]<sub>i</sub> ). Additionally, the starved cells acquire a significantly enhanced capacity to undergo a progesterone-induced acrosome reaction. Electrophysiological measurements show that CatSper channel remains active in starvation conditi","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Apr","modification":"2025-04-22T09:20:25.792Z","creation":"2025-04-05T22:59:28.423Z"},"accession":"S-EPMC8441833","cross_references":{"pubmed":["33742713"],"doi":["10.1096/fj.202100122R","10.1096/fj.202100122r"]}}