{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Xian J"],"funding":["the International Scientific and Technological Cooperation Projects of Xinjiang Production and Construction Corps","the National Natural Science Foundation of China"],"pagination":["489"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8460197"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["14(1)"],"pubmed_abstract":["<h4>Background</h4>Cystic echinococcosis (CE) is a serious parasitic zoonosis caused by the larvae of the tapeworm Echinococcus granulosus. The development of an effective vaccine is one of the most promising strategies for controlling CE.<h4>Methods</h4>The E. granulosus 3-hydroxyacyl-CoA dehydrogenase (EgHCDH) gene was cloned and expressed in Escherichia coli. The distribution of EgHCDH in protoscoleces (PSCs) and adult worms was analyzed using immunofluorescence. The transcript levels of EgHCDH in PSCs and adult worms were analyzed using quantitative real-time reverse transcription PCR (RT-qPCR). The immune protective effects of the rEgHCDH were evaluated.<h4>Results</h4>The 924-bp open reading frame sequence of EgHCDH, which encodes a protein of approximately 34 kDa, was obtained. RT-q"],"journal":["Parasites & vectors"],"pubmed_title":["Molecular characterization and immune protection of the 3-hydroxyacyl-CoA dehydrogenase gene in Echinococcus granulosus."],"pmcid":["PMC8460197"],"funding_grant_id":["2021BC008","31860701","2020BC007"],"pubmed_authors":["Wang N","Zhang Y","Guo X","Xian J","Zhao P","Ma X","Bo X","Meng J","Wang Z"],"additional_accession":[]},"is_claimable":false,"name":"Molecular characterization and immune protection of the 3-hydroxyacyl-CoA dehydrogenase gene in Echinococcus granulosus.","description":"<h4>Background</h4>Cystic echinococcosis (CE) is a serious parasitic zoonosis caused by the larvae of the tapeworm Echinococcus granulosus. The development of an effective vaccine is one of the most promising strategies for controlling CE.<h4>Methods</h4>The E. granulosus 3-hydroxyacyl-CoA dehydrogenase (EgHCDH) gene was cloned and expressed in Escherichia coli. The distribution of EgHCDH in protoscoleces (PSCs) and adult worms was analyzed using immunofluorescence. The transcript levels of EgHCDH in PSCs and adult worms were analyzed using quantitative real-time reverse transcription PCR (RT-qPCR). The immune protective effects of the rEgHCDH were evaluated.<h4>Results</h4>The 924-bp open reading frame sequence of EgHCDH, which encodes a protein of approximately 34 kDa, was obtained. RT-q","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Sep","modification":"2025-06-01T02:21:18.088Z","creation":"2022-02-11T11:20:02.869Z"},"accession":"S-EPMC8460197","cross_references":{"pubmed":["34556147"],"doi":["10.1186/s13071-021-05001-z"]}}