{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wu T"],"funding":["NICHD NIH HHS","National Institutes of Health"],"pagination":["100142"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8461376"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["20"],"pubmed_abstract":["Chromatin-associated RNAs have diverse roles in the nucleus. However, their mechanisms of action are poorly understood, in part because of the inability to identify proteins that specifically associate with chromatin-bound RNAs. Here, we address this problem for a subset of chromatin-associated RNAs that form R-loops-RNA-DNA hybrid structures that include a displaced strand of ssDNA. R-loops generally form cotranscriptionally and have important roles in regulation of gene expression, immunoglobulin class switching, and other processes. However, unresolved R-loops can lead to DNA damage and chromosome instability. To identify factors that may bind and regulate R-loop accumulation or mediate R-loop-dependent functions, we used a comparative immunoprecipitation/MS approach, with and without R"],"journal":["Molecular & cellular proteomics : MCP"],"pubmed_title":["Characterization of R-Loop-Interacting Proteins in Embryonic Stem Cells Reveals Roles in rRNA Processing and Gene Expression."],"pmcid":["PMC8461376"],"funding_grant_id":["R01 HD093783","R01HD072122","R01 HD072122","R01HD093783"],"pubmed_authors":["Chu F","Fazzio TG","Wu T","Nance J"],"additional_accession":[]},"is_claimable":false,"name":"Characterization of R-Loop-Interacting Proteins in Embryonic Stem Cells Reveals Roles in rRNA Processing and Gene Expression.","description":"Chromatin-associated RNAs have diverse roles in the nucleus. However, their mechanisms of action are poorly understood, in part because of the inability to identify proteins that specifically associate with chromatin-bound RNAs. Here, we address this problem for a subset of chromatin-associated RNAs that form R-loops-RNA-DNA hybrid structures that include a displaced strand of ssDNA. R-loops generally form cotranscriptionally and have important roles in regulation of gene expression, immunoglobulin class switching, and other processes. However, unresolved R-loops can lead to DNA damage and chromosome instability. To identify factors that may bind and regulate R-loop accumulation or mediate R-loop-dependent functions, we used a comparative immunoprecipitation/MS approach, with and without R","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021","modification":"2026-05-08T13:10:43.25Z","creation":"2022-02-11T11:29:32.643Z"},"accession":"S-EPMC8461376","cross_references":{"pubmed":["34478875"],"doi":["10.1016/j.mcpro.2021.100142"]}}