{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wellner A"],"funding":["Gordon and Betty Moore Foundation (Gordon E. and Betty I. Moore Foundation)","NIAID NIH HHS","Vallee Foundation (Bert L. & N. Kuggie Vallee Foundation)","Massachusetts Consortium on Pathogenesis Readiness","Gordon and Betty Moore Foundation","UC | University of California, Irvine","Vallee Foundation","NCI NIH HHS","U.S. Department of Health & Human Services | National Institutes of Health (NIH)","UC | University of California, Irvine (UC Irvine)","NIGMS NIH HHS","U.S. Department of Health & Human Services | National Institutes of Health","NIH HHS"],"pagination":["1057-1064"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8463502"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["17(10)"],"pubmed_abstract":["The predominant approach for antibody generation remains animal immunization, which can yield exceptionally selective and potent antibody clones owing to the powerful evolutionary process of somatic hypermutation. However, animal immunization is inherently slow, not always accessible and poorly compatible with many antigens. Here, we describe 'autonomous hypermutation yeast surface display' (AHEAD), a synthetic recombinant antibody generation technology that imitates somatic hypermutation inside engineered yeast. By encoding antibody fragments on an error-prone orthogonal DNA replication system, surface-displayed antibody repertoires continuously mutate through simple cycles of yeast culturing and enrichment for antigen binding to produce high-affinity clones in as little as two weeks. We "],"journal":["Nature chemical biology"],"pubmed_title":["Rapid generation of potent antibodies by autonomous hypermutation in yeast."],"pmcid":["PMC8463502"],"funding_grant_id":["Scholars Award","DP5OD021345","1R35GM136297","COVID-19 Basic, Translational and Clinical Research Fund","R01AI146779","T32 GM007753","T32 AI007245","T32 GM008313","1R01CA260415","Inventors Fellowship","R01 AI146779","T32GM007753","R01 CA260415","DP2 GM119163","R35 GM136297","DP5 OD021345"],"pubmed_authors":["Liu CC","Schmidt AG","Abraham J","Wingler LM","Kruse AC","Hu VJ","Wellner A","Hauser BM","Feldman J","Caradonna TM","Marks DS","Gilman MSA","Nguyen KM","Shin JE","Clements JR","McMahon C","Clark S","Ho MH"],"additional_accession":[]},"is_claimable":false,"name":"Rapid generation of potent antibodies by autonomous hypermutation in yeast.","description":"The predominant approach for antibody generation remains animal immunization, which can yield exceptionally selective and potent antibody clones owing to the powerful evolutionary process of somatic hypermutation. However, animal immunization is inherently slow, not always accessible and poorly compatible with many antigens. Here, we describe 'autonomous hypermutation yeast surface display' (AHEAD), a synthetic recombinant antibody generation technology that imitates somatic hypermutation inside engineered yeast. By encoding antibody fragments on an error-prone orthogonal DNA replication system, surface-displayed antibody repertoires continuously mutate through simple cycles of yeast culturing and enrichment for antigen binding to produce high-affinity clones in as little as two weeks. We ","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Oct","modification":"2026-05-31T15:56:11.243Z","creation":"2025-04-04T12:46:21.377Z"},"accession":"S-EPMC8463502","cross_references":{"pubmed":["34168368"],"doi":["10.1038/s41589-021-00832-4"]}}