biostudies-literatureDi Domenico MDeutsche Forschungsgemeinschaft2310https://www.ebi.ac.uk/biostudies/studies/S-EPMC8467068biostudies-literatureUnknown10(9)Profilin functions have been discussed in numerous cellular processes, including actin polymerization. One puzzling aspect is the concomitant expression of more than one profilin isoform in most tissues. In neuronal precursors and in neurons, profilin 1 and profilin 2 are co-expressed, but their specific and redundant functions in brain morphogenesis are still unclear. Using a conditional knockout mouse model to inactivate both profilins in the developing CNS, we found that threshold levels of profilin are necessary for the maintenance of the neuronal stem-cell compartment and the generation of the differentiated neurons, irrespective of the specific isoform. During embryonic development, profilin 1 is more abundant than profilin 2; consequently, modulation of profilin 1 levels resulted inCellsSpecificity and Redundancy of Profilin 1 and 2 Function in Brain Development and Neuronal Structure.PMC8467068SPP1464SFB1089Pilo Boyl PWitke WDi Domenico MJokwitz MfalseSpecificity and Redundancy of Profilin 1 and 2 Function in Brain Development and Neuronal Structure.Profilin functions have been discussed in numerous cellular processes, including actin polymerization. One puzzling aspect is the concomitant expression of more than one profilin isoform in most tissues. In neuronal precursors and in neurons, profilin 1 and profilin 2 are co-expressed, but their specific and redundant functions in brain morphogenesis are still unclear. Using a conditional knockout mouse model to inactivate both profilins in the developing CNS, we found that threshold levels of profilin are necessary for the maintenance of the neuronal stem-cell compartment and the generation of the differentiated neurons, irrespective of the specific isoform. During embryonic development, profilin 1 is more abundant than profilin 2; consequently, modulation of profilin 1 levels resulted in2021-01-01T00:00:00Z2021 Sep2025-04-21T16:08:58.859Z2022-02-11T11:35:35.353ZS-EPMC84670683457195910.3390/cells10092310