{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Janickova Z"],"funding":["Vedecká Grantová Agentúra MŠVVaŠ SR a SAV"],"pagination":["5704"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8467227"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["26(18)"],"pubmed_abstract":["This study brings a detailed bioinformatics analysis of fungal and chloride-dependent α-amylases from the family GH13. Overall, 268 α-amylase sequences were retrieved from subfamilies GH13_1 (39 sequences), GH13_5 (35 sequences), GH13_15 (28 sequences), GH13_24 (23 sequences), GH13_32 (140 sequences) and GH13_42 (3 sequences). Eight conserved sequence regions (CSRs) characteristic for the family GH13 were identified in all sequences and respective sequence logos were analysed in an effort to identify unique sequence features of each subfamily. The main emphasis was given on the subfamily GH13_32 since it contains both fungal α-amylases and their bacterial chloride-activated counterparts. In addition to in silico analysis focused on eventual ability to bind the chloride anion, the property "],"journal":["Molecules (Basel, Switzerland)"],"pubmed_title":["In Silico Analysis of Fungal and Chloride-Dependent α-Amylases within the Family GH13 with Identification of Possible Secondary Surface-Binding Sites."],"pmcid":["PMC8467227"],"funding_grant_id":["2/0146/21"],"pubmed_authors":["Janecek S","Janickova Z"],"additional_accession":[]},"is_claimable":false,"name":"In Silico Analysis of Fungal and Chloride-Dependent α-Amylases within the Family GH13 with Identification of Possible Secondary Surface-Binding Sites.","description":"This study brings a detailed bioinformatics analysis of fungal and chloride-dependent α-amylases from the family GH13. Overall, 268 α-amylase sequences were retrieved from subfamilies GH13_1 (39 sequences), GH13_5 (35 sequences), GH13_15 (28 sequences), GH13_24 (23 sequences), GH13_32 (140 sequences) and GH13_42 (3 sequences). Eight conserved sequence regions (CSRs) characteristic for the family GH13 were identified in all sequences and respective sequence logos were analysed in an effort to identify unique sequence features of each subfamily. The main emphasis was given on the subfamily GH13_32 since it contains both fungal α-amylases and their bacterial chloride-activated counterparts. In addition to in silico analysis focused on eventual ability to bind the chloride anion, the property ","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Sep","modification":"2026-04-08T06:55:59.083Z","creation":"2022-02-11T11:41:04.848Z"},"accession":"S-EPMC8467227","cross_references":{"pubmed":["34577174"],"doi":["10.3390/molecules26185704"]}}