biostudies-literatureDoulias PTNIDA NIH HHSNIA NIH HHSNIMH NIH HHSNINDS NIH HHSNational Institutes of Health367-378https://www.ebi.ac.uk/biostudies/studies/S-EPMC8477648biostudies-literatureUnknown27(3)In the brain, both HIV-1 and methamphetamine (meth) use result in increases in oxidative and nitrosative stress. This redox stress is thought to contribute to the pathogenesis of HIV-associated neurocognitive disorder (HAND) and further worsening cognitive activity in the setting of drug abuse. One consequence of such redox stress is aberrant protein S-nitrosylation, derived from nitric oxide, which may disrupt normal protein activity. Here, we report an improved, mass spectrometry-based technique to assess S-nitrosylated protein in human postmortem brains using selective enrichment of S-nitrosocysteine residues with an organomercury resin. The data show increasing S-nitrosylation of tricarboxylic acid (TCA) enzymes in the setting of HAND and HAND/meth use compared with HIV+ control brainsJournal of neurovirologyTCA cycle metabolic compromise due to an aberrant S-nitrosoproteome in HIV-associated neurocognitive disorder with methamphetamine use.PMC8477648R01 AG056259U24 MH100928RF1 AG057409R01 AG061845DP1 DA041722R01 NS086890Scott HIschiropoulos HMcKercher SRAlbertolle MLipton SADoulias PTNakamura TDeal ASultan AfalseTCA cycle metabolic compromise due to an aberrant S-nitrosoproteome in HIV-associated neurocognitive disorder with methamphetamine use.In the brain, both HIV-1 and methamphetamine (meth) use result in increases in oxidative and nitrosative stress. This redox stress is thought to contribute to the pathogenesis of HIV-associated neurocognitive disorder (HAND) and further worsening cognitive activity in the setting of drug abuse. One consequence of such redox stress is aberrant protein S-nitrosylation, derived from nitric oxide, which may disrupt normal protein activity. Here, we report an improved, mass spectrometry-based technique to assess S-nitrosylated protein in human postmortem brains using selective enrichment of S-nitrosocysteine residues with an organomercury resin. The data show increasing S-nitrosylation of tricarboxylic acid (TCA) enzymes in the setting of HAND and HAND/meth use compared with HIV+ control brains2021-01-01T00:00:00Z2021 Jun2025-04-19T18:16:57.651Z2025-02-18T23:35:48.12ZS-EPMC84776483387641410.1007/s13365-021-00970-4