biostudies-literatureWang PJiangsu Province Natural Science FundsNational Natural Science FundsNational Key Research and Development Program of China442https://www.ebi.ac.uk/biostudies/studies/S-EPMC8480016biostudies-literatureUnknown21(1)<h4>Background</h4>Stone-hardening stage is crucial to the development of grape seed and berry quality. A significant body of evidence supports the important roles of MicroRNAs in grape-berry development, but their specific molecular functions during grape stone-hardening stage remain unclear.<h4>Results</h4>Here, a total of 161 conserved and 85 species-specific miRNAs/miRNAs* (precursor) were identified in grape berries at stone-hardening stage using Solexa sequencing. Amongst them, 30 VvmiRNAs were stone-hardening stage-specific, whereas 52 exhibited differential expression profiles during berry development, potentially participating in the modulation of berry development as verified by their expression patterns. GO and KEGG pathway analysis showed that 13 VvmiRNAs might be involved in tBMC plant biologyIdentification of miRNAs-mediated seed and stone-hardening regulatory networks and their signal pathway of GA-induced seedless berries in grapevine (V. vinifera L.).PMC8480016319723732018YFD1000106BK2018131831672131BK2016058731801809Liu ZWang PFang JAbdelrahman MWang CZhang XXuan XWang WSu ZWang XJiu SfalseIdentification of miRNAs-mediated seed and stone-hardening regulatory networks and their signal pathway of GA-induced seedless berries in grapevine (V. vinifera L.).<h4>Background</h4>Stone-hardening stage is crucial to the development of grape seed and berry quality. A significant body of evidence supports the important roles of MicroRNAs in grape-berry development, but their specific molecular functions during grape stone-hardening stage remain unclear.<h4>Results</h4>Here, a total of 161 conserved and 85 species-specific miRNAs/miRNAs* (precursor) were identified in grape berries at stone-hardening stage using Solexa sequencing. Amongst them, 30 VvmiRNAs were stone-hardening stage-specific, whereas 52 exhibited differential expression profiles during berry development, potentially participating in the modulation of berry development as verified by their expression patterns. GO and KEGG pathway analysis showed that 13 VvmiRNAs might be involved in t2021-01-01T00:00:00Z2021 Sep2026-05-09T06:21:19.168Z2022-02-11T11:45:13.389ZS-EPMC84800163458791410.1186/s12870-021-03188-y