biostudies-literatureSun-Wada GHMinistry of Education, Culture, Sports, Science and TechnologyIndividual Research Grants in Doshisha Women’s College of Liberal ArtsJapan Society for the Promotion of Science19355https://www.ebi.ac.uk/biostudies/studies/S-EPMC8481250biostudies-literatureUnknown11(1)The endocytic compartments keep their interior acidic through the inward flow of protons and anions from the cytosol. Acidification is mediated by a proton pump known as vacuolar-type ATPase (V-ATPase) and transporters conferring anion conductance to the organellar membrane. In this study, we analysed the phenotype of mouse embryos lacking the V-ATPase c-subunit. The mutant embryos differentiated embryonic epithelial tissues, primitive endoderm, epiblast, and extraembryonic ectoderm; however, the organisation of these epithelia was severely affected. The apical-basal polarity in the visceral endoderm layer was not properly established in the mutant embryos, resulting in abnormal epithelial morphology. Thus, the function of V-ATPase is imperative for the establishment and/or maintenance of epithelial cell polarity, which is required for early embryogenesis.Scientific reportsVacuolar-type proton ATPase is required for maintenance of apicobasal polarity of embryonic visceral endoderm.PMC8481250JP19K05950JP21K06553JP20H05323Tabata HWada YSun-Wada GHfalseVacuolar-type proton ATPase is required for maintenance of apicobasal polarity of embryonic visceral endoderm.The endocytic compartments keep their interior acidic through the inward flow of protons and anions from the cytosol. Acidification is mediated by a proton pump known as vacuolar-type ATPase (V-ATPase) and transporters conferring anion conductance to the organellar membrane. In this study, we analysed the phenotype of mouse embryos lacking the V-ATPase c-subunit. The mutant embryos differentiated embryonic epithelial tissues, primitive endoderm, epiblast, and extraembryonic ectoderm; however, the organisation of these epithelia was severely affected. The apical-basal polarity in the visceral endoderm layer was not properly established in the mutant embryos, resulting in abnormal epithelial morphology. Thus, the function of V-ATPase is imperative for the establishment and/or maintenance of epithelial cell polarity, which is required for early embryogenesis.2021-01-01T00:00:00Z2021 Sep2024-10-15T20:55:40.847Z2022-02-11T11:38:35.523ZS-EPMC84812503458857910.1038/s41598-021-98952-3