biostudies-literatureMangsbo SMScience for Life LaboratoryVetenskapsrådetLundblad family foundationSvenska Sällskapet för Medicinsk ForskningRegion stockholmFamiljen Erling-Perssons StiftelseKnut och Alice Wallenbergs StiftelseHjärt-LungfondenJonas & Christina af Jochnick foundatione0258041https://www.ebi.ac.uk/biostudies/studies/S-EPMC8483319biostudies-literatureUnknown16(9)Numerous assays evaluating serological and cellular responses have been developed to characterize immune responses against SARS-CoV-2. Serological assays are both cost- and time-effective compared to cellular assays, but cellular immune responses may provide a diagnostic value to determine previous SARS-CoV-2 infection in seronegative individuals. However, potential cross-reactive T cell responses stemming from prior encounters with human coronaviruses (HCoVs) may affect assay specificity. In this study, we evaluated the specificity and sensitivity of a SARS-CoV-2 IFN-γ Release Assay (IGRA) based on the FluoroSpot method employing commercially available SARS-CoV-2-specific peptide pools, as well as an in-house designed SARS-CoV-2 peptide pool restricted to 5 amino acid stretches or less alPloS oneAn evaluation of a FluoroSpot assay as a diagnostic tool to determine SARS-CoV-2 specific T cell responses.PMC84833192020-058802020-05782201904682018-02552Lord MHober SLindsay RJernbom Falk AThalin CMarking ULauren IBuggert MChristoffersson GPhillipson MKlingstrom JMangsbo SMHavervall SNilsson PDonnes PfalseAn evaluation of a FluoroSpot assay as a diagnostic tool to determine SARS-CoV-2 specific T cell responses.Numerous assays evaluating serological and cellular responses have been developed to characterize immune responses against SARS-CoV-2. Serological assays are both cost- and time-effective compared to cellular assays, but cellular immune responses may provide a diagnostic value to determine previous SARS-CoV-2 infection in seronegative individuals. However, potential cross-reactive T cell responses stemming from prior encounters with human coronaviruses (HCoVs) may affect assay specificity. In this study, we evaluated the specificity and sensitivity of a SARS-CoV-2 IFN-γ Release Assay (IGRA) based on the FluoroSpot method employing commercially available SARS-CoV-2-specific peptide pools, as well as an in-house designed SARS-CoV-2 peptide pool restricted to 5 amino acid stretches or less al2021-01-01T00:00:00Z20212026-03-16T15:46:19.139Z2022-02-11T11:39:29.792ZS-EPMC84833193459191810.1371/journal.pone.0258041