biostudies-literatureKay HMedical Research CouncilRoyal SocietyWellcome TrustBiotechnology and Biological Sciences Research Council1147https://www.ebi.ac.uk/biostudies/studies/S-EPMC8484446biostudies-literatureUnknown4(1)The cellular landscape changes dramatically over the course of a 24 h day. The proteome responds directly to daily environmental cycles and is additionally regulated by the circadian clock. To quantify the relative contribution of diurnal versus circadian regulation, we mapped proteome dynamics under light:dark cycles compared with constant light. Using Ostreococcus tauri, a prototypical eukaryotic cell, we achieved 85% coverage, which allowed an unprecedented insight into the identity of proteins that facilitate rhythmic cellular functions. The overlap between diurnally- and circadian-regulated proteins was modest and these proteins exhibited different phases of oscillation between the two conditions. Transcript oscillations were generally poorly predictive of protein oscillations, in whiCommunications biologyDeep-coverage spatiotemporal proteome of the picoeukaryote Ostreococcus tauri reveals differential effects of environmental and endogenous 24-hour rhythms.PMC8484446BB/M010996/1MC_UP_1201/4MC_U105184326UF160685Grunewald EPeak-Chew SYStangherlin AGil SKay HFeord HKO'Neill JSvan Ooijen GfalseDeep-coverage spatiotemporal proteome of the picoeukaryote Ostreococcus tauri reveals differential effects of environmental and endogenous 24-hour rhythms.The cellular landscape changes dramatically over the course of a 24 h day. The proteome responds directly to daily environmental cycles and is additionally regulated by the circadian clock. To quantify the relative contribution of diurnal versus circadian regulation, we mapped proteome dynamics under light:dark cycles compared with constant light. Using Ostreococcus tauri, a prototypical eukaryotic cell, we achieved 85% coverage, which allowed an unprecedented insight into the identity of proteins that facilitate rhythmic cellular functions. The overlap between diurnally- and circadian-regulated proteins was modest and these proteins exhibited different phases of oscillation between the two conditions. Transcript oscillations were generally poorly predictive of protein oscillations, in whi2021-01-01T00:00:00Z2021 Sep2025-04-04T10:21:45.805Z2025-04-04T10:21:45.805ZS-EPMC84844463459397510.1038/s42003-021-02680-3