{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kaczmarczyk JA"],"funding":["CCR NIH HHS","NCI NIH HHS"],"pagination":["2022-2038"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8487723"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12(20)"],"pubmed_abstract":["Lung cancer is the leading cause of cancer-related deaths in the USA and worldwide. Yet, about 95% of new drug candidates validated in preclinical phase eventually fail in clinical trials. Such a high attrition rate is attributed mostly to the inability of conventional two-dimensionally (2D) cultured cancer cells to mimic native three-dimensional (3D) growth of malignant cells in human tumors. To ascertain phenotypical differences between these two distinct culture conditions, we carried out a comparative proteomic analysis of a membrane fraction obtained from 3D- and 2D-cultured NSCLC model cell line NCI-H23. This analysis revealed a map of 1,166 (24%) protein species regulated in culture dependent manner, including differential regulation of a subset of cell surface-based CD molecules. W"],"journal":["Oncotarget"],"pubmed_title":["Comparative microsomal proteomics of a model lung cancer cell line NCI-H23 reveals distinct differences between molecular profiles of 3D and 2D cultured cells."],"pmcid":["PMC8487723"],"funding_grant_id":["HHSN261200800001E","HHSN261200800001C"],"pubmed_authors":["Simona C","Chan KC","Roberts RR","Van Wagoner CM","Kaczmarczyk JA","Luke BT","Felder RA","Saul RG","Blonder J"],"additional_accession":[]},"is_claimable":false,"name":"Comparative microsomal proteomics of a model lung cancer cell line NCI-H23 reveals distinct differences between molecular profiles of 3D and 2D cultured cells.","description":"Lung cancer is the leading cause of cancer-related deaths in the USA and worldwide. Yet, about 95% of new drug candidates validated in preclinical phase eventually fail in clinical trials. Such a high attrition rate is attributed mostly to the inability of conventional two-dimensionally (2D) cultured cancer cells to mimic native three-dimensional (3D) growth of malignant cells in human tumors. To ascertain phenotypical differences between these two distinct culture conditions, we carried out a comparative proteomic analysis of a membrane fraction obtained from 3D- and 2D-cultured NSCLC model cell line NCI-H23. This analysis revealed a map of 1,166 (24%) protein species regulated in culture dependent manner, including differential regulation of a subset of cell surface-based CD molecules. W","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Sep","modification":"2026-05-09T01:40:53.984Z","creation":"2022-02-11T11:53:33.707Z"},"accession":"S-EPMC8487723","cross_references":{"pubmed":["34611477"],"doi":["10.18632/oncotarget.28072"]}}