biostudies-literatureAlexander KLNational Institute of Allergy and Infectious DiseasesCrohn&apos;s and Colitis Foundation of AmericaKenneth Rainin FoundationNIAID NIH HHSCrohn's & Colitis FoundationNational Institutes of HealthU.S. Department of Veterans AffairsCSRD VA522-535.e6https://www.ebi.ac.uk/biostudies/studies/S-EPMC8489510biostudies-literatureUnknown161(2)<h4>Background and aims</h4>Crohn's disease and ulcerative colitis are characterized by dysregulated adaptive immune responses to the microbiota in genetically susceptible individuals, but the specificity of these responses remains largely undefined. Therefore, we developed a microbiota antigen microarray to characterize microbial antibody reactivity, particularly to human-derived microbiota flagellins, in inflammatory bowel disease.<h4>Methods</h4>Sera from healthy volunteers (n = 87) at the University of Alabama at Birmingham and from patients recruited from the Kirklin Clinic of University of Alabama at Birmingham Hospital, including patients with Crohn's disease (n = 152) and ulcerative colitis (n = 170), were individually probed against microbiota bacterial flagellins of both mouse anGastroenterologyHuman Microbiota Flagellins Drive Adaptive Immune Responses in Crohn's Disease.PMC848951032655I01 CX001530T32 AI007051523929CX0001530AI007051KRA000010Alexander KLReif MDuck LWZhao QRosenberg AFMannon PJElson COfalseHuman Microbiota Flagellins Drive Adaptive Immune Responses in Crohn's Disease.<h4>Background and aims</h4>Crohn's disease and ulcerative colitis are characterized by dysregulated adaptive immune responses to the microbiota in genetically susceptible individuals, but the specificity of these responses remains largely undefined. Therefore, we developed a microbiota antigen microarray to characterize microbial antibody reactivity, particularly to human-derived microbiota flagellins, in inflammatory bowel disease.<h4>Methods</h4>Sera from healthy volunteers (n = 87) at the University of Alabama at Birmingham and from patients recruited from the Kirklin Clinic of University of Alabama at Birmingham Hospital, including patients with Crohn's disease (n = 152) and ulcerative colitis (n = 170), were individually probed against microbiota bacterial flagellins of both mouse an2021-01-01T00:00:00Z2021 Aug2026-05-08T11:29:45.291Z2025-04-19T06:02:51.344ZS-EPMC84895103384498710.1053/j.gastro.2021.03.064