{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Onder S"],"funding":["National Institute of Environmental Health Sciences","NIEHS NIH HHS","Türkiye Bilimsel ve Teknolojik AraÅ?tirma Kurumu","National Cancer Institute","NCI NIH HHS"],"pagination":["4728-4745"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8491160"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["20(10)"],"pubmed_abstract":["Chronic low-dose exposure to organophosphorus pesticides is associated with the risk of neurodegenerative disease. The mechanism of neurotoxicity is independent of acetylcholinesterase inhibition. Adducts on tyrosine, lysine, threonine, and serine can occur after exposure to organophosphorus pesticides, the most stable being adducts on tyrosine. Rabbit monoclonal 1C6 to diethoxyphosphate-modified tyrosine (depY) was created by single B cell cloning. The amino acid sequence and binding constant (<i>K</i><sub>d</sub> 3.2 × 10<sup>-8</sup> M) were determined. Cultured human neuroblastoma SH-SY5Y and mouse neuroblastoma N2a cells incubated with a subcytotoxic dose of 10 μM chlorpyrifos oxon contained depY-modified proteins detected by monoclonal 1C6 on Western blots. depY-labeled peptides from"],"journal":["Journal of proteome research"],"pubmed_title":["Rabbit Antidiethoxyphosphotyrosine Antibody, Made by Single B Cell Cloning, Detects Chlorpyrifos Oxon-Modified Proteins in Cultured Cells and Immunopurifies Modified Peptides for Mass Spectrometry."],"pmcid":["PMC8491160"],"funding_grant_id":["P30 CA036727","R21 ES030132","P30CA036727","1R21ES030132-01A1","SBAG-318S259"],"pubmed_authors":["van Grol M","Tacal O","Yerramalla U","Fidder A","Noort D","Onder S","Xiao G","Lockridge O","Schopfer LM"],"additional_accession":[]},"is_claimable":false,"name":"Rabbit Antidiethoxyphosphotyrosine Antibody, Made by Single B Cell Cloning, Detects Chlorpyrifos Oxon-Modified Proteins in Cultured Cells and Immunopurifies Modified Peptides for Mass Spectrometry.","description":"Chronic low-dose exposure to organophosphorus pesticides is associated with the risk of neurodegenerative disease. The mechanism of neurotoxicity is independent of acetylcholinesterase inhibition. Adducts on tyrosine, lysine, threonine, and serine can occur after exposure to organophosphorus pesticides, the most stable being adducts on tyrosine. Rabbit monoclonal 1C6 to diethoxyphosphate-modified tyrosine (depY) was created by single B cell cloning. The amino acid sequence and binding constant (<i>K</i><sub>d</sub> 3.2 × 10<sup>-8</sup> M) were determined. Cultured human neuroblastoma SH-SY5Y and mouse neuroblastoma N2a cells incubated with a subcytotoxic dose of 10 μM chlorpyrifos oxon contained depY-modified proteins detected by monoclonal 1C6 on Western blots. depY-labeled peptides from","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Oct","modification":"2026-05-10T05:42:01.399Z","creation":"2022-02-11T11:53:21.389Z"},"accession":"S-EPMC8491160","cross_references":{"pubmed":["34469172"],"doi":["10.1021/acs.jproteome.1c00383"]}}