biostudies-literatureWatanabe YAmerican Heart AssociationNIDDK NIH HHSNHLBI NIH HHSUehara Memorial Foundation5827-5837https://www.ebi.ac.uk/biostudies/studies/S-EPMC8491561biostudies-literatureUnknown34(4)Reactive oxygen species (ROS) increase during adipogenesis and in obesity. Oxidants react with cysteine residues of proteins to form glutathione (GSH) adducts, S-glutathionylation, that are selectively removed by glutaredoxin-1 (Glrx). We have previously reported that Glrx knockout mice had increased protein S-glutathionylation and developed obesity by an unknown mechanism. In this study, we demonstrated that 3T3L1 adipocytes differentiation increased ROS and protein S-glutathionylation. Glrx ablation elevated protein S-glutathionylation and lipid content in 3T3L1 cells. Glrx replenishment decreased the lipid content of Glrx KO 3T3L1 cells. Glrx KO also increased protein expression and protein S-glutathionylation of the adipogenic transcription factor CCAAT enhancer-binding protein (C/EBP)FASEB journal : official publication of the Federation of American Societies for Experimental BiologyProtein S-glutathionylation stimulate adipogenesis by stabilizing C/EBPβ in 3T3L1 cells.PMC8491561R01 DK103750R01 HL13301316GRNT27660006Bachschmid MMWatanabe YFujioka DNakamura KKugiyama KWatanabe KNakamura TUematsu MMatsui RfalseProtein S-glutathionylation stimulate adipogenesis by stabilizing C/EBPβ in 3T3L1 cells.Reactive oxygen species (ROS) increase during adipogenesis and in obesity. Oxidants react with cysteine residues of proteins to form glutathione (GSH) adducts, S-glutathionylation, that are selectively removed by glutaredoxin-1 (Glrx). We have previously reported that Glrx knockout mice had increased protein S-glutathionylation and developed obesity by an unknown mechanism. In this study, we demonstrated that 3T3L1 adipocytes differentiation increased ROS and protein S-glutathionylation. Glrx ablation elevated protein S-glutathionylation and lipid content in 3T3L1 cells. Glrx replenishment decreased the lipid content of Glrx KO 3T3L1 cells. Glrx KO also increased protein expression and protein S-glutathionylation of the adipogenic transcription factor CCAAT enhancer-binding protein (C/EBP)2020-01-01T00:00:00Z2020 Apr2025-04-04T22:43:30.588Z2022-02-11T11:47:17.362ZS-EPMC84915613214112710.1096/fj.201902575R10.1096/fj.201902575r