biostudies-literatureZiffra RSNIMH NIH HHSNHGRI NIH HHSNIH HHSNIGMS NIH HHS205-213https://www.ebi.ac.uk/biostudies/studies/S-EPMC8494642biostudies-literatureUnknown598(7879)During mammalian development, differences in chromatin state coincide with cellular differentiation and reflect changes in the gene regulatory landscape¹. In the developing brain, cell fate specification and topographic identity are important for defining cell identity² and confer selective vulnerabilities to neurodevelopmental disorders³. Here, to identify cell-type-specific chromatin accessibility patterns in the developing human brain, we used a single-cell assay for transposase accessibility by sequencing (scATAC-seq) in primary tissue samples from the human forebrain. We applied unbiased analyses to identify genomic loci that undergo extensive cell-type- and brain-region-specific changes in accessibility during neurogenesis, and an integrative analysisNatureSingle-cell epigenomics reveals mechanisms of human cortical development.PMC84946421K99 MH1171655U41 HG002371-19U41 HG002371RF1 MH132662K99 MH117165U24 HG002371U01 MH114825T32 GM007175R01 MH109907U01 MH116438U01 MH115747R01 MH125516R01 MH123178R01 MH101221RF1 MH121268Ziffra RSNowakowski TJHaeussler MKim CNPrzytycki PFWilfert ATurner TNPollard KSKeough KCShin DRoss JMKreimer ABogdanoff DCasella AMEichler EEAhituv NAment SAfalseSingle-cell epigenomics reveals mechanisms of human cortical development.During mammalian development, differences in chromatin state coincide with cellular differentiation and reflect changes in the gene regulatory landscape¹. In the developing brain, cell fate specification and topographic identity are important for defining cell identity² and confer selective vulnerabilities to neurodevelopmental disorders³. Here, to identify cell-type-specific chromatin accessibility patterns in the developing human brain, we used a single-cell assay for transposase accessibility by sequencing (scATAC-seq) in primary tissue samples from the human forebrain. We applied unbiased analyses to identify genomic loci that undergo extensive cell-type- and brain-region-specific changes in accessibility during neurogenesis, and an integrative analysis2021-01-01T00:00:00Z2021 Oct2026-05-07T22:25:24.501Z2025-04-19T08:25:16.537ZS-EPMC84946423461606010.1038/s41586-021-03209-8