{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Nighot M"],"funding":["NIDDK NIH HHS","Crohn's & Colitis Foundation of America","National Institute of Diabetes and Digestive and Kidney Diseases","National Institutes of Health","NIH HHS"],"pagination":["1751-1765"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8495490"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["15(10)"],"pubmed_abstract":["<h4>Background and aims</h4>Matrix metalloproteinases [MMPs] play an important role in extracellular matrix regulation during cell growth and wound healing. Increased expression of MMP-12 [human macrophage elastase] has been reported in inflammatory bowel disease [IBD] which is characterised by the loss of epithelial tight junction [TJ] barrier function and an excessive inflammatory response. The aim of this study was to investigate the role of MMP-12 in intestinal TJ barrier function and inflammation.<h4>Methods</h4>Wild type [WT] and MMP-12-/- mice were subjected to experimental acute or chronic dextran sodium sulphate [DSS] colitis. The mouse colonic permeability was measured in vivo by recycling perfusion of the entire colon and ex vivo by Ussing chamber studies.<h4>Results</h4>DSS adm"],"journal":["Journal of Crohn's & colitis"],"pubmed_title":["Matrix Metalloproteinase MMP-12 Promotes Macrophage Transmigration Across Intestinal Epithelial Tight Junctions and Increases Severity of Experimental Colitis."],"pmcid":["PMC8495490"],"funding_grant_id":["R01 DK114024","DK-106072","R01 DK106072","DK100562","694583","DK114024","K01 DK100562"],"pubmed_authors":["Suchanec E","Gregory A","Nighot M","Ganapathy AS","Shapiro S","Saha K","Nighot P","Ma T","Castillo EF"],"additional_accession":[]},"is_claimable":false,"name":"Matrix Metalloproteinase MMP-12 Promotes Macrophage Transmigration Across Intestinal Epithelial Tight Junctions and Increases Severity of Experimental Colitis.","description":"<h4>Background and aims</h4>Matrix metalloproteinases [MMPs] play an important role in extracellular matrix regulation during cell growth and wound healing. Increased expression of MMP-12 [human macrophage elastase] has been reported in inflammatory bowel disease [IBD] which is characterised by the loss of epithelial tight junction [TJ] barrier function and an excessive inflammatory response. The aim of this study was to investigate the role of MMP-12 in intestinal TJ barrier function and inflammation.<h4>Methods</h4>Wild type [WT] and MMP-12-/- mice were subjected to experimental acute or chronic dextran sodium sulphate [DSS] colitis. The mouse colonic permeability was measured in vivo by recycling perfusion of the entire colon and ex vivo by Ussing chamber studies.<h4>Results</h4>DSS adm","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Oct","modification":"2025-04-22T09:57:30.049Z","creation":"2025-04-05T23:23:19.233Z"},"accession":"S-EPMC8495490","cross_references":{"pubmed":["33836047"],"doi":["10.1093/ecco-jcc/jjab064"]}}