biostudies-literatureNighot MNIDDK NIH HHSCrohn's & Colitis Foundation of AmericaNational Institute of Diabetes and Digestive and Kidney DiseasesNational Institutes of HealthNIH HHS1751-1765https://www.ebi.ac.uk/biostudies/studies/S-EPMC8495490biostudies-literatureUnknown15(10)<h4>Background and aims</h4>Matrix metalloproteinases [MMPs] play an important role in extracellular matrix regulation during cell growth and wound healing. Increased expression of MMP-12 [human macrophage elastase] has been reported in inflammatory bowel disease [IBD] which is characterised by the loss of epithelial tight junction [TJ] barrier function and an excessive inflammatory response. The aim of this study was to investigate the role of MMP-12 in intestinal TJ barrier function and inflammation.<h4>Methods</h4>Wild type [WT] and MMP-12-/- mice were subjected to experimental acute or chronic dextran sodium sulphate [DSS] colitis. The mouse colonic permeability was measured in vivo by recycling perfusion of the entire colon and ex vivo by Ussing chamber studies.<h4>Results</h4>DSS admJournal of Crohn's & colitisMatrix Metalloproteinase MMP-12 Promotes Macrophage Transmigration Across Intestinal Epithelial Tight Junctions and Increases Severity of Experimental Colitis.PMC8495490R01 DK114024DK-106072R01 DK106072DK100562694583DK114024K01 DK100562Suchanec EGregory ANighot MGanapathy ASShapiro SSaha KNighot PMa TCastillo EFfalseMatrix Metalloproteinase MMP-12 Promotes Macrophage Transmigration Across Intestinal Epithelial Tight Junctions and Increases Severity of Experimental Colitis.<h4>Background and aims</h4>Matrix metalloproteinases [MMPs] play an important role in extracellular matrix regulation during cell growth and wound healing. Increased expression of MMP-12 [human macrophage elastase] has been reported in inflammatory bowel disease [IBD] which is characterised by the loss of epithelial tight junction [TJ] barrier function and an excessive inflammatory response. The aim of this study was to investigate the role of MMP-12 in intestinal TJ barrier function and inflammation.<h4>Methods</h4>Wild type [WT] and MMP-12-/- mice were subjected to experimental acute or chronic dextran sodium sulphate [DSS] colitis. The mouse colonic permeability was measured in vivo by recycling perfusion of the entire colon and ex vivo by Ussing chamber studies.<h4>Results</h4>DSS adm2021-01-01T00:00:00Z2021 Oct2025-04-22T09:57:30.049Z2025-04-05T23:23:19.233ZS-EPMC84954903383604710.1093/ecco-jcc/jjab064