biostudies-literatureWang GNational Cancer InstituteNCI NIH HHS301.e17-301.e28https://www.ebi.ac.uk/biostudies/studies/S-EPMC8501538biostudies-literatureUnknown39(5)<h4>Purpose</h4>We evaluated the prognostic value of 10 putative tumor markers by immunohistochemistry in a large multi-institutional cohort of patients with locally advanced urothelial cancer of the bladder (UCB) with the aim to validate their clinical value and to harmonize protocols for their evaluation.<h4>Materials and methods</h4>Primary tumor specimens from 576 patients with pathologic (p)T3 UCB were collected from 24 institutions in North America and Europe. Three replicate 0.6-mm core diameter samples were collected for the construction of a tissue microarray (TMA). Immunohistochemistry (IHC) for 10 previously described tumor markers was performed and scored at 3 laboratories independently according to a standardized protocol. Associations between marker positivity and freedom froUrologic oncologyPrognostic markers in pT3 bladder cancer: A study from the international bladder cancer tissue microarray project.PMC8501538P30 CA016672P50 CA091846R01 CA071921Dinney CPMinner SCote RJJi LWang GBlack PCCordon-Cardo CGroshen SSchmitz-Drager BHawes DSauter GCzerniak BWaldman FGoebell PJfalsePrognostic markers in pT3 bladder cancer: A study from the international bladder cancer tissue microarray project.<h4>Purpose</h4>We evaluated the prognostic value of 10 putative tumor markers by immunohistochemistry in a large multi-institutional cohort of patients with locally advanced urothelial cancer of the bladder (UCB) with the aim to validate their clinical value and to harmonize protocols for their evaluation.<h4>Materials and methods</h4>Primary tumor specimens from 576 patients with pathologic (p)T3 UCB were collected from 24 institutions in North America and Europe. Three replicate 0.6-mm core diameter samples were collected for the construction of a tissue microarray (TMA). Immunohistochemistry (IHC) for 10 previously described tumor markers was performed and scored at 3 laboratories independently according to a standardized protocol. Associations between marker positivity and freedom fro2021-01-01T00:00:00Z2021 May2025-04-05T14:05:32.235Z2025-04-05T14:05:32.235ZS-EPMC85015383356353910.1016/j.urolonc.2021.01.021