{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Alinezhadbalalami N"],"funding":["NIBIB NIH HHS","NIAID NIH HHS","NCI NIH HHS"],"pagination":["107886"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8504467"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["142"],"pubmed_abstract":["Expansion of cytotoxic T lymphocytes (CTLs) is a crucial step in almost all cancer immunotherapeutic methods. Current techniques for expansion of tumor-reactive CTLs present major limitations. This study introduces a novel method to effectively produce and expand tumor-activated CTLs using high-voltage pulsed electric fields. We hypothesize that utilizing high-voltage pulsed electric fields may be an ideal method to activate and expand CTLs due to their non-thermal celldeath mechanism. Tumor cells were subjected to high-frequency irreversible electroporation (HFIRE) with various electric field magnitudes (1250, 2500 V/cm) and pulse widths (1, 5, and 10 µs), or irreversible electroporation (IRE) at 1250 V/cm. The treated tumor cells were subsequently cocultured with CD4+ and CD8+ T cells al"],"journal":["Bioelectrochemistry (Amsterdam, Netherlands)"],"pubmed_title":["Generation of Tumor-activated T cells Using Electroporation."],"pmcid":["PMC8504467"],"funding_grant_id":["R01 CA213423","R03 AI151494","R21 EB028429"],"pubmed_authors":["Graybill PM","Verbridge SS","Imran KM","Allen IC","Alinezhadbalalami N","Davalos RV"],"additional_accession":[]},"is_claimable":false,"name":"Generation of Tumor-activated T cells Using Electroporation.","description":"Expansion of cytotoxic T lymphocytes (CTLs) is a crucial step in almost all cancer immunotherapeutic methods. Current techniques for expansion of tumor-reactive CTLs present major limitations. This study introduces a novel method to effectively produce and expand tumor-activated CTLs using high-voltage pulsed electric fields. We hypothesize that utilizing high-voltage pulsed electric fields may be an ideal method to activate and expand CTLs due to their non-thermal celldeath mechanism. Tumor cells were subjected to high-frequency irreversible electroporation (HFIRE) with various electric field magnitudes (1250, 2500 V/cm) and pulse widths (1, 5, and 10 µs), or irreversible electroporation (IRE) at 1250 V/cm. The treated tumor cells were subsequently cocultured with CD4+ and CD8+ T cells al","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Dec","modification":"2025-04-25T20:54:56.948Z","creation":"2025-04-06T08:33:17.698Z"},"accession":"S-EPMC8504467","cross_references":{"pubmed":["34303065"],"doi":["10.1016/j.bioelechem.2021.107886"]}}