biostudies-literatureAlinezhadbalalami NNIBIB NIH HHSNIAID NIH HHSNCI NIH HHS107886https://www.ebi.ac.uk/biostudies/studies/S-EPMC8504467biostudies-literatureUnknown142Expansion of cytotoxic T lymphocytes (CTLs) is a crucial step in almost all cancer immunotherapeutic methods. Current techniques for expansion of tumor-reactive CTLs present major limitations. This study introduces a novel method to effectively produce and expand tumor-activated CTLs using high-voltage pulsed electric fields. We hypothesize that utilizing high-voltage pulsed electric fields may be an ideal method to activate and expand CTLs due to their non-thermal celldeath mechanism. Tumor cells were subjected to high-frequency irreversible electroporation (HFIRE) with various electric field magnitudes (1250, 2500 V/cm) and pulse widths (1, 5, and 10 µs), or irreversible electroporation (IRE) at 1250 V/cm. The treated tumor cells were subsequently cocultured with CD4+ and CD8+ T cells alBioelectrochemistry (Amsterdam, Netherlands)Generation of Tumor-activated T cells Using Electroporation.PMC8504467R01 CA213423R03 AI151494R21 EB028429Graybill PMVerbridge SSImran KMAllen ICAlinezhadbalalami NDavalos RVfalseGeneration of Tumor-activated T cells Using Electroporation.Expansion of cytotoxic T lymphocytes (CTLs) is a crucial step in almost all cancer immunotherapeutic methods. Current techniques for expansion of tumor-reactive CTLs present major limitations. This study introduces a novel method to effectively produce and expand tumor-activated CTLs using high-voltage pulsed electric fields. We hypothesize that utilizing high-voltage pulsed electric fields may be an ideal method to activate and expand CTLs due to their non-thermal celldeath mechanism. Tumor cells were subjected to high-frequency irreversible electroporation (HFIRE) with various electric field magnitudes (1250, 2500 V/cm) and pulse widths (1, 5, and 10 µs), or irreversible electroporation (IRE) at 1250 V/cm. The treated tumor cells were subsequently cocultured with CD4+ and CD8+ T cells al2021-01-01T00:00:00Z2021 Dec2025-04-25T20:54:56.948Z2025-04-06T08:33:17.698ZS-EPMC85044673430306510.1016/j.bioelechem.2021.107886