biostudies-literatureMatos GSBLRD VACNPqFAPERJNIAID NIH HHSDepartment of Veterans AffairsNIH158983https://www.ebi.ac.uk/biostudies/studies/S-EPMC8512607biostudies-literatureUnknown1866(9)SBF (Swi4/Swi6 Binding Factor) complex is a crucial regulator of G1/S transition in Saccharomyces cerevisiae. Here, we show that SBF complex is required for myriocin resistance, an inhibitor of sphingolipid synthesis. This phenotype was not shared with MBF complex mutants nor with deletion of the Swi4p downstream targets, CLN1/CLN2. Based on data mining results, we selected putative Swi4p targets related to sphingolipid metabolism and studied their gene transcription as well as metabolite levels during progression of the cell cycle. Genes which encode key enzymes for the synthesis of long chain bases (LCBs) and ceramides were periodically transcribed during the mitotic cell cycle, having a peak at G1/S, and required SWI4 for full transcription at this stage. In addition, HPLC-MS/MS data inBiochimica et biophysica acta. Molecular and cell biology of lipidsRegulation of sphingolipid synthesis by the G1/S transcription factor Swi4.PMC8512607R01 AI116420R01 AI136934R01 AI125770I01 BX002624Fernandes CMMatos GSMontero-Lomeli MMasuda CADel Poeta MDasilva DMadeira JBfalseRegulation of sphingolipid synthesis by the G1/S transcription factor Swi4.SBF (Swi4/Swi6 Binding Factor) complex is a crucial regulator of G1/S transition in Saccharomyces cerevisiae. Here, we show that SBF complex is required for myriocin resistance, an inhibitor of sphingolipid synthesis. This phenotype was not shared with MBF complex mutants nor with deletion of the Swi4p downstream targets, CLN1/CLN2. Based on data mining results, we selected putative Swi4p targets related to sphingolipid metabolism and studied their gene transcription as well as metabolite levels during progression of the cell cycle. Genes which encode key enzymes for the synthesis of long chain bases (LCBs) and ceramides were periodically transcribed during the mitotic cell cycle, having a peak at G1/S, and required SWI4 for full transcription at this stage. In addition, HPLC-MS/MS data in2021-01-01T00:00:00Z2021 Sep2025-04-04T09:37:37.692Z2025-04-04T09:37:37.692ZS-EPMC85126073406225510.1016/j.bbalip.2021.158983