biostudies-literatureZhang YMinistry of Science and Technology of ChinaUSTC research fundsfundamental research funds for the central universitiesNational Natural Science Foundation of China20359https://www.ebi.ac.uk/biostudies/studies/S-EPMC8514580biostudies-literatureUnknown11(1)Previous studies indicated that the P-body components, CGH-1 and EDC-3 may play a crucial role in the regulation of lifespan in Caenorhabditis elegans. Homo sapiens DDX6 or Saccharomyces cerevisiae Dhh1p (CGH-1 in C. elegans) could form complexes with EDC3 (Edc3p in yeast), respectively, which is significant for translation inhibition and mRNA decay. However, it is currently unclear how CGH-1 can be recognized by EDC-3 in C. elegans. Here, we provided structural and biochemical insights into the interaction between CGH-1 and EDC-3. Combined with homology modeling, mutation, and ITC assays, we uncovered an interface between CGH-1 RecA2 domain and EDC-3 FDF-FEK. Additionally, GST-pulldown and co-localization experiments confirmed the interaction between CGH-1 and EDC-3 in vitro and in vivo. Scientific reportsInsight into the interaction between the RNA helicase CGH-1 and EDC-3 and its implications.PMC851458031870760WK20700001452019YFA050840331970669KY2070000075Yang KZhang YHong JWang KShi YfalseInsight into the interaction between the RNA helicase CGH-1 and EDC-3 and its implications.Previous studies indicated that the P-body components, CGH-1 and EDC-3 may play a crucial role in the regulation of lifespan in Caenorhabditis elegans. Homo sapiens DDX6 or Saccharomyces cerevisiae Dhh1p (CGH-1 in C. elegans) could form complexes with EDC3 (Edc3p in yeast), respectively, which is significant for translation inhibition and mRNA decay. However, it is currently unclear how CGH-1 can be recognized by EDC-3 in C. elegans. Here, we provided structural and biochemical insights into the interaction between CGH-1 and EDC-3. Combined with homology modeling, mutation, and ITC assays, we uncovered an interface between CGH-1 RecA2 domain and EDC-3 FDF-FEK. Additionally, GST-pulldown and co-localization experiments confirmed the interaction between CGH-1 and EDC-3 in vitro and in vivo. 2021-01-01T00:00:00Z2021 Oct2026-05-09T01:25:01.925Z2022-02-11T12:10:31.722ZS-EPMC85145803464593110.1038/s41598-021-99919-0