biostudies-literatureChen XNatural Science Foundation of Ningbo1383-1398https://www.ebi.ac.uk/biostudies/studies/S-EPMC8518580biostudies-literatureUnknown22(11)The Chinese wheat mosaic virus (CWMV) genome consists of two positive-strand RNAs that are required for CWMV replication and translation. The eukaryotic translation elongation factor (eEF1A) is crucial for the elongation of protein translation in eukaryotes. Here, we show that silencing eEF1A expression in Nicotiana benthamiana plants by performing virus-induced gene silencing can greatly reduce the accumulation of CWMV genomic RNAs, whereas overexpression of eEF1A in plants increases the accumulation of CWMV genomic RNAs. In vivo and in vitro assays showed that eEF1A does not interact with CWMV RNA-dependent RNA polymerase. Electrophoretic mobility shift assays revealed that eEF1A can specifically bind to the 3'-untranslated region (UTR) of CWMV genomic RNAs. By performing mutational analMolecular plant pathologyBinding between elongation factor 1A and the 3'-UTR of Chinese wheat mosaic virus is crucial for virus infection.PMC85185802019A610415Yang JXu MLi JZhang TChen JZhang HLiao QChen XHe LfalseBinding between elongation factor 1A and the 3'-UTR of Chinese wheat mosaic virus is crucial for virus infection.The Chinese wheat mosaic virus (CWMV) genome consists of two positive-strand RNAs that are required for CWMV replication and translation. The eukaryotic translation elongation factor (eEF1A) is crucial for the elongation of protein translation in eukaryotes. Here, we show that silencing eEF1A expression in Nicotiana benthamiana plants by performing virus-induced gene silencing can greatly reduce the accumulation of CWMV genomic RNAs, whereas overexpression of eEF1A in plants increases the accumulation of CWMV genomic RNAs. In vivo and in vitro assays showed that eEF1A does not interact with CWMV RNA-dependent RNA polymerase. Electrophoretic mobility shift assays revealed that eEF1A can specifically bind to the 3'-untranslated region (UTR) of CWMV genomic RNAs. By performing mutational anal2021-01-01T00:00:00Z2021 Nov2026-05-08T19:51:50.278Z2024-11-07T02:59:31.712ZS-EPMC85185803440550710.1111/mpp.13120